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M6018

Sigma-Aldrich

4-Methylumbelliferyl β-D-cellobioside

glucanase substrate, fluorogenic, ≥98.00% (TLC), powder

Synonym(s):

4-Methylumbelliferyl beta-D-cellobioside

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100 MG
¥28,300
500 MG
¥97,100

¥28,300

Available to ship onApril 07, 2025Details

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100 MG
¥28,300
500 MG
¥97,100

About This Item

Empirical Formula (Hill Notation):
C22H28O13
CAS Number:
72626-61-0
Molecular Weight:
500.45
EC Number:
276-744-7
MDL number:
MFCD00063279
UNSPSC Code:
12352204
PubChem Substance ID:
24897067
NACRES:
NA.32

¥28,300

Available to ship onApril 07, 2025Details

Product Name

4-Methylumbelliferyl β-D-cellobioside, glucanase substrate

Quality Level

200

Assay

≥98.00% (TLC)

form

powder

solubility

water: 19.60-20.40 mg/mL, clear, colorless to faintly yellow

storage temp.

2-8°C

SMILES string

CC1=CC(=O)Oc2cc(O[C@@H]3O[C@H](CO)[C@@H](O[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)ccc12

InChI

1S/C22H28O13/c1-8-4-14(25)32-11-5-9(2-3-10(8)11)31-21-19(30)17(28)20(13(7-24)34-21)35-22-18(29)16(27)15(26)12(6-23)33-22/h2-5,12-13,15-24,26-30H,6-7H2,1H3/t12-,13-,15-,16+,17-,18-,19-,20-,21-,22+/m1/s1

InChI key

PRTGXBPFDYMIJH-MKQZUAMYSA-N

Application

4-Methylumbelliferyl β-D-cellobioside has been used as a substrate:
  • to assay cellulase from A. niger[1]
  • to determine the extracellular enzyme activity for 1,4-β-cellobiohydrolase[2]
  • to assay the activity of bound enzyme, endoglucanase I (EGIc) Trichoderma reesei class II hydrophobins (HFBI)[3]

Biochem/physiol Actions

4-Methylumbelliferyl β-D-cellobioside is used as a substrate for cellulase assays. It is mainly used for activity staining of cellulases in polyacrylamide gels.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

M6018-100MG:
M6018-VAR:
M6018-500MG:
M6018-500MG-PW:
M6018-100MG-PW:
M6018-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number
0000430619
0000430619
0000401301
0000401301
0000327307

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Reconciling opposing soil processes in row-crop agroecosystems via soil functional zone management
Williams A, et al.
Agriculture, Ecosystems & Environment, 236, 99-107 (2017)
Methylxanthine Drugs Are Chitinase Inhibitors: Investigation of Inhibition and Binding Modes
Rao FV, et al.
Chemistry & Biology, 12(9), 973-980 (2005)
G P Xue et al.
Journal of general microbiology, 138(11), 2397-2403 (1992-11-01)
A plant polysaccharide hydrolase cDNA, designated celD, was isolated from a cDNA library of the rumen fungus Neocallimastix patriciarum. The enzyme encoded by celD had endoglucanase, cellobiohydrolase and xylanase activities. Deletion analysis revealed that celD cDNA can be truncated to
M Pedraza-Reyes et al.
Archives of microbiology, 168(4), 321-327 (1997-09-23)
The gram-negative bacterium Myxobacter sp. AL-1 produces chitosanase-cellulase activity that is maximally excreted during the stationary phase of growth. Carboxymethylcellulase zymogram analysis revealed that the enzymatic activity was correlated with two bands of 32 and 35 kDa. Ion-exchange-chromatography-enriched preparations of
K H Jung et al.
Biochemistry and molecular biology international, 44(2), 283-292 (1998-04-08)
A Clostridium thermocellum xylanase gene, designated xynX, was cloned in Escherichia coli and was categorized a novel gene as a result of the comparison of restriction patterns of the C. thermocellum xylanase genes so far reported. The xynX gene encodes
View All Related Papers

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