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Safety Information

06-1363

Sigma-Aldrich

Anti-SRp30a (SF2) Antibody

from rabbit

Synonym(s):

serine/arginine-rich splicing factor 1, pre-mRNA-splicing factor SF2, P33 subunit, SR splicing factor 1, splicing factor 2, splicing factor, arginine/serine-rich 1, Alternative-splicing factor 1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, rat, human

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SRSF1(6426)

General description

SRp30a, also known as SF2, ASF-1 (alternative splicing factor 1), is a member of the highly conserved family of SR proteins, a group of serine/arginine-rich domain-containing proteins that play a critical role in regulated and constitutive splicing of precursor mRNA. The SR family of proteins is characterized as having one to two RNA recognition motifs located in the n-terminal sequence, and an RS domain within the C-terminus that is rich in serine/arginine dipeptide repeats. ASF-1 has an inhibitory role in exon skipping, in order to maintain splicing accuracy and to monitor alternative splicing. Studies have shown that ASF-1 plays a role in the regulation of caspase-9 alternative splicing as well as being a critical component for ceramide to function as a mediator of alternative splicing for the same caspase.

Immunogen

Linear peptide corresponding to human SRp30a.

Application

Immunocytochemistry Analysis:
1:500 dilution from a representative lot detected SRp30a in NIH/3T3 cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins
Use Anti-SRp30a (SF2) Antibody (rabbit polyclonal antibody) validated in WB, ICC to detect SRp30a (SF2) also known as serine/arginine-rich splicing factor 1, pre-mRNA-splicing factor SF2- P33 subunit, SR splicing factor 1.

Quality

Evaluated by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: 1:500 dilution of this antibody detected SRp30a on 10 µg of NIH/3T3 cell lysate.

Target description

~40 kDa observed. An uncharacterized band at ~ 50 kDa has been observed in some lysates.

Physical form

Format: Purified
Protein A
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
NIH/3T3 cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

06-1363:


Certificates of Analysis (COA)

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