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322415

Sigma-Aldrich

Methanol

≥99.8%, anhydrous, suitable for tissue processing

Synonym(s):

Methyl alcohol

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About This Item

Linear Formula:
CH3OH
CAS Number:
Molecular Weight:
32.04
Beilstein:
1098229
EC Number:
MDL number:
UNSPSC Code:
12352104
PubChem Substance ID:

product name

Methanol, anhydrous, 99.8%

grade

anhydrous

Quality Level

vapor density

1.11 (vs air)

vapor pressure

410 mmHg ( 50 °C)
97.68 mmHg ( 20 °C)

Assay

99.8%

form

liquid

autoignition temp.

725 °F

expl. lim.

36 %

technique(s)

tissue processing: suitable

impurities

<0.002% water
<0.005% water (100 mL pkg)

evapn. residue

<0.0003%

refractive index

n20/D 1.329 (lit.)

bp

64.7 °C (lit.)

mp

−98 °C (lit.)

density

0.791 g/mL at 25 °C (lit.)

format

neat

SMILES string

CO

InChI

1S/CH4O/c1-2/h2H,1H3

InChI key

OKKJLVBELUTLKV-UHFFFAOYSA-N

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General description

Methanol is an industrial organic solvent commonly used in organic synthesis. It is being considered as a potential hydrogen source in fuel cell technology due to its high H/C ratio, low propensity for soot generation, relatively low reforming temperature and its liquid state at room temperature. The olefins (ethylene or propylene) formed from methanol via MTO (methanol-to-olefins) process, can be an alternative to oil and gas to produce hydrocarbon fuels.

Application

Methanol has been used for fixing the tissues during preparation of thin giemsa stained blood smears. It has been used in the purification step during the synthesis of pyocyanin, a toxic compound found in Pseudomonas aeruginosa.
Methanol has been used in following applications:
  • Used for the synthesis and purification of pyocyanin, spectrophotometric estimation of pyocyanin concentration, and the calculation of pyocyanin molar concentration using spectrophotometer absorption data.
  • Used as a component of staining solution used in karyotyping.
  • Used as a component of staining solution to stain cell colonies in colony formation assay.

Other Notes

Pure-Pac® II containers require the Micromatic MacroValve coupler for dispensing solvents, Z560723.

Legal Information

Pure-Pac is a registered trademark of Merck KGaA, Darmstadt, Germany

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

Target Organs

Eyes,Central nervous system

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

49.5 °F - closed cup

Flash Point(C)

9.7 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

FSL

Group 4: Flammable liquids
Alcohols
Hazardous rank II

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

322415-PZ:
322415-18L:4548173134994
322415-VAR:
322415-BULK:
322415-2L:4548173135021
322415-100ML:4548173134970
322415-200L:4548173135014
322415-200L-P2:
322415-18L-P1:
322415-20L:
322415-UC-20L-P2:
322415-20L-P2:
QR-028-1L:
322415-8L:
322415-12X100ML:4548173134987
322415-1L:4548173135007
322415-250ML:
322415-900ML:
322415-200L-P2-SA:
322415-6L:
322415-4X2L:4548173135038
322415-6X1L:4548173135045
322415-1250L-P1:
322415-RSAMPLE:


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Matthew P Horning et al.
Lab on a chip, 14(12), 2040-2046 (2014-05-02)
A paper microfluidic cartridge for the automated staining of malaria parasites (Plasmodium) with acridine orange prior to microscopy is presented. The cartridge enables simultaneous, sub-minute generation of both thin and thick smears of acridine orange stained parasites. Parasites are stained
Rajkumar Cheluvappa
MethodsX, 1, 67-73 (2014-01-01)
Preparation of the toxin pyocyanin from the bacterium Pseudomonas aeruginosa is an exacting procedure. Pyocyanin is expensive to commercially purchase. The sellers do not give out the extraction procedure. Classically, pyocyanin preparation involves complicated multi-step P. aeruginosa culturing and solvent
Generation of Embryonic Stem Cells in Rabbits.
Methods in Molecular Biology, 1341, 49-66 (2016)
Standardized chemical synthesis of Pseudomonas aeruginosa pyocyanin.
Cheluvappa R.
Gastroenterologia y hepatologia, 22, 1350-1351 (2007)
Beyond oil and gas: the methanol economy.
Olah GA.
Angewandte Chemie (International Edition in English), 44(18), 2636-2639 (2005)

Articles

Substances are said to be miscible in one another if they dissolve to form a uniform solution. Bookmark or download our miscibility table for common lab solvents.

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