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797006

Sigma-Aldrich

In Vitro Protein Expression (iPE-SS) Kit for disulfide-containing proteins

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.12

usage

Stable Isotope Labeling
1 extractions

Quality Level

200

technique(s)

bio NMR: suitable
protein expression: suitable

storage temp.

−70°C

Application

This is a kit which is improved for the synthesis of extracellular secretory proteins such as cytokines and antibodies. This kit is suitable for the synthesis of stable isotope labeled disulfide-containing proteins.
This kit has been developed under license from RIKEN, incorporating their proprietary, advanced cell-free protein synthesizing technology into a kit dedicated to stable isotope labeling.

Packaging

Stable isotope labeled amino acid mixtures are not included in this kit. Please use the separately sold stable isotope labeled amino acids mixtures. (Prod. No. 767972, 767964, and 771031)
This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

Kit Components Also Available Separately

Product No.
Description
SDS
  • Internal Solution-SS
  • External Solution-SS
  • Control DNA (pUC-BAP)
  • L-Glutathione oxidized disodium salt
  • L-Glutathione reduced
  • Dialysis Cup

Hazard Statements

H412

Precautionary Statements

P273 - P501

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

10 - Combustible liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Please refer to KIT Component information

PRTR

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FSL

Please refer to KIT Component information

ISHL Indicated Name

Please refer to KIT Component information

ISHL Notified Names

Please refer to KIT Component information

Cartagena Act

Please refer to KIT Component information

JAN Code

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Certificates of Analysis (COA)

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Jun Yokoyama et al.
Analytical biochemistry, 411(2), 223-229 (2011-01-25)
During recent years, the targets of protein structure analysis using nuclear magnetic resonance spectroscopy have become larger and more complicated. As a result, a complete and precise stable isotope labeling technique has been desired. A cell-free protein synthesis system is
Takayoshi Matsuda et al.
Biochemical and biophysical research communications, 431(2), 296-301 (2013-01-08)
Many important therapeutic targets are secreted proteins with multiple disulfide bonds, such as antibodies, cytokines, hormones, and proteases. The preparation of these proteins for structural and functional analyses using cell-based expression systems still suffers from several issues, such as inefficiency

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