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905089

Sigma-Aldrich

In Vitro Protein Expression (iPE) Kit II

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.12

technique(s)

bio NMR: suitable
protein expression: suitable

Quality Level

200

storage temp.

−70°C

Related Categories

Application

iPE-Quick Kit (prod.no. 767824) is available and intended for the confirmation of target protein expression utilizing E. Coli extract before the use of this iPE kit.
This is a protein synthesis system that utilizes E. coli cell extract. It allows easy and efficient protein expression by adding circular or linear DNA as the template DNA, which enables transcription of mRNA with T7 RNA polymerase.
This kit has been developed under license from RIKEN, incorporating their proprietary, advanced cell-free protein synthesizing technology into a kit dedicated to stable isotope labeling.
This kit is not intended to be used for disulfide-containing proteins. For disulfide-containing proteins, refer to prod.no. 797006.

Packaging

For information on pricing and availability, please contact Stable Isotopes Customer Service.
Stable isotope labeled amino acid mixtures are not included in this kit. Please use separately-sold stable isotope labeled amino acids mixtures (prod. no. 767972, 767964, 771031).

Storage Class Code

11 - Combustible Solids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Please refer to KIT Component information

PRTR

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FSL

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ISHL Indicated Name

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ISHL Notified Names

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Cartagena Act

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JAN Code

キットコンポーネントの情報を参照してください

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Eiko Seki et al.
Analytical biochemistry, 377(2), 156-161 (2008-04-01)
Cell-free protein synthesis has become one of the standard methods for protein expression. One of the major advantages of this method is that PCR-amplified linear DNA fragments can be directly used as templates for protein synthesis. The productivity of cell-free
William C Yang et al.
Biotechnology progress, 28(2), 413-420 (2012-01-26)
Escherichia coli cell-free protein synthesis (CFPS) uses E. coli extracts to make active proteins in vitro. The basic CFPS reaction mixture is comprised of four main reagent components: (1) energy source and CFPS chemicals, (2) DNA encoding the protein of
Takayoshi Matsuda et al.
Journal of biomolecular NMR, 37(3), 225-229 (2007-01-24)
Cell-free protein synthesis is suitable for stable-isotope labeling of proteins for NMR analysis. The Escherichia coli cell-free system containing potassium acetate for efficient translation (KOAc system) is usually used for stable-isotope labeling, although it is less productive than other systems.
Takashi Yabuki et al.
Journal of structural and functional genomics, 8(4), 173-191 (2008-01-02)
A two-step PCR method has been developed for the robust, high-throughput production of linear templates ready for cell-free protein synthesis. The construct made from the cDNA expresses a target protein region with N- and/or C-terminal tags. The procedure consists only
Takanori Kigawa et al.
Journal of structural and functional genomics, 5(1-2), 63-68 (2004-07-21)
As structural genomics and proteomics research has become popular, the importance of cell-free protein synthesis systems has been realized for high-throughput expression. Our group has established a high-throughput pipeline for protein sample preparation for structural genomics and proteomics by using
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