- A new method based on supercritical fluid extraction for polyacetylenes and polyenes from Echinacea pallida (Nutt.) Nutt. roots.
A new method based on supercritical fluid extraction for polyacetylenes and polyenes from Echinacea pallida (Nutt.) Nutt. roots.
Journal of pharmaceutical and biomedical analysis (2017-08-26)
Massimo Tacchini, Antonella Spagnoletti, Virginia Brighenti, Francesco Pio Prencipe, Stefania Benvenuti, Gianni Sacchetti, Federica Pellati
PMID28841426
ABSTRACT
The genus Echinacea (Asteraceae) includes species traditionally used in phytotherapy. Among them, Echinacea pallida (Nutt.) Nutt. root extracts are characterized by a representative antiproliferative activity, due to the presence of acetylenic compounds. In this study, supercritical fluid extraction (SFE) was applied and compared with conventional Soxhlet extraction (SE) in order to obtain a bioactive extract highly rich in polyacetylenes and polyenes from E. pallida roots. The composition of the extracts was monitored by means of HPLC-UV/DAD and HPLC-ESI-MS
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Colonna per HPLC Ascentis® Express C18, 2,7 μm, 2.7 μm particle size, L × I.D. 5 cm × 4.6 mm
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Colonna per HPLC Ascentis® Express C18, 2,7 μm, 2.7 μm particle size, L × I.D. 10 cm × 3 mm
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Colonna per HPLC Ascentis® Express C18, 2,7 μm, 2.7 μm particle size, L × I.D. 15 cm × 3 mm
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Colonna per HPLC Ascentis® Express C18, 2,7 μm, 2.7 μm particle size, L × I.D. 15 cm × 2.1 mm
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Colonna per HPLC Ascentis® Express C18, 2,7 μm, 2.7 μm particle size, L × I.D. 10 cm × 2.1 mm
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Cartuccia di guardia per Ascentis® Express C18, 2,7 micron, 2.7 μm particle size, L × I.D. 5 mm × 4.6 mm, pkg of 3 ea
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Colonna per HPLC Ascentis® Express C18, 2,7 μm, 2.7 μm particle size, L × I.D. 3 cm × 2.1 mm
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Cartuccia di guardia per Ascentis® Express C18, 2,7 micron, 2.7 μm particle size, L × I.D. 5 mm × 2.1 mm, pkg of 3 ea
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