Ubiquitin Activating Enzyme (E1) is responsible for the first step in ubiquitin-protein isopeptide bond formation. E1 catalyzes the activation of the C-terminal carboxyl group of ubiquitin by forming a high-energy thioester bond in an ATP-dependent manner.
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Solution in 50 mM HEPES, pH 8.0, 50 mM NaCl and 1mM TCEP
International journal of biological sciences, 16(6), 1059-1070 (2020-03-07)
Cullin 4A and 4B (CUL4A and 4B) function as oncogenes in colorectal cancer (CRC) cells. Both of them conservatively associate with DNA damage-binding protein 1 (DDB1) and DDB1-CUL4-associated factor 4 (DCAF4) to form Cullin-RING E3 ligases known as CRL4DCAF4, which
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