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U0251

Sigma-Aldrich

Urease from Canavalia ensiformis (Jack bean)

Type C-3, powder, ≥600,000 units/g solid

Sinonimo/i:

Jack bean urease, Urea amidohydrolase

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About This Item

Numero CAS:
9002-13-5
Classificazione EC (Enzyme Commission):
3.5.1.5 (BRENDA, IUBMB)
Numero CE:
232-656-0
Numero MDL:
MFCD00070858
Codice UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.54

Origine biologica

Canavalia ensiformis

Tipo

Type C-3

Forma fisica

powder

Attività specifica

≥600,000 units/g solid

PM

~544620 Da

Purificato mediante

crystallization

Temperatura di conservazione

−20°C

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Descrizione generale

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Urease is a nickel-dependent metalloenzyme , found in various plants, bacteria, fungi, and algae. It contains two distinct subunits-UreA(26.5 kDa) and UreB (60.3–61.6 kDa).

Azioni biochim/fisiol

Urease aids in the bioavailability of nitrogen in plants. It is implicated in defense mechanism and exhibits insecticidal activity. In addition, urease exhibits antifungal activity by retarding growth and affecting membrane integrity of filamentous fungi and yeasts.
Urease was shown to induce vacuolation in Hela cells, an effect dependent on the ability of urease to convert urea to ammonia.

Definizione di unità

One micromolar unit will liberate 1.0 μmole of NH3 from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)

Pittogrammi

Health hazardExclamation mark
GHS08,GHS07

Avvertenze

Danger

Indicazioni di pericolo

H315,H319,H334,H335

Classi di pericolo

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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J Ryan Feathers et al.
Journal of structural biology: X, 5, 100047-100047 (2021-04-06)
The resolution of cryo-EM reconstructions is fundamentally limited by the Nyquist frequency, which is half the sampling frequency of the detector and depends upon the magnification used. In principle, super-resolution imaging should enable reconstructions to surpass the physical Nyquist limit
T L Cover et al.
Infection and immunity, 59(4), 1264-1270 (1991-04-01)
Concentrated broth culture supernatants from 50 to 60% of Helicobacter pylori strains induce eukaryotic cell vacuolation in vitro. A quantitative assay for cell vacuolation was developed on the basis of the rapid uptake of visibly vacuolated HeLa cells was significantly
Barbara Krajewska et al.
Plant physiology and biochemistry : PPB, 43(7), 651-658 (2005-07-19)
A pH-variation study of jack bean (Canavalia ensiformis) urease steady-state kinetic parameters and of the inhibition constant of boric acid, a urease competitive inhibitor, was performed using both noninhibitory organic (MES, HEPES and CHES) and inhibitory inorganic (phosphate) buffers, in
J Jessy et al.
The Biochemical journal, 277 ( Pt 3), 693-696 (1991-08-01)
Recent studies showed that hyperammonaemia caused many of the metabolic changes in portacaval-shunted rats, a model of hepatic encephalopathy. These changes included a depression in the cerebral metabolic rate of glucose (CMRGlc), an indication of decreased brain function. 2. The
N E Dixon et al.
Science (New York, N.Y.), 191(4232), 1144-1150 (1976-03-19)
In an attempt to understand the role of nickel in jack bean urease (1), we turned to a variety of other enzymes important in the utilization, production, or transfer of ammonia. We found several, including the L-histidine and L-phenylalanine ammonialyases
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