This material has a molecular weight of 34.6 kDa. The molar extinction coefficient at 280 nm is approximately 6.11 × 10⁴ M⁻¹ cm⁻¹, calculated from the reported E¹%₂₈₀ value of 17.65 and a molecular weight of 34,600 g/mol.
T7902
Thermolysin from Geobacillus stearothermophilus
powder, BioReagent, 30-350 units/mg protein (E1%/280), suitable for cell culture
Sinonimo/i:
Protease from Geobacillus stearothermophilus, Thermophilic-bacterial protease
Autenticati per visualizzare i prezzi organizzativi e contrattuali.
Scegli un formato
Cambia visualizzazione
| Voi/SKU | Disponibilità | Prezzo |
|---|---|---|
25 mg | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 57,70 € |
100 mg | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 176,00 € |
250 mg | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 365,00 € |
Informazioni su questo articolo
Numero CAS:
UNSPSC Code:
12352204
NACRES:
NA.75
EC Number:
232-973-4
MDL number:
Numero CE:
eCl@ss:
32160410
Specific activity:
30-350 units/mg protein (E1%/280)
Biological source:
Geobacillus stearothermophilus
57,70 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Servizio Tecnico
Hai bisogno di aiuto? Il nostro team di scienziati qualificati è a tua disposizione.
Permettici di aiutartibiological source
Geobacillus stearothermophilus
Quality Segment
product line
BioReagent
form
powder
specific activity
30-350 units/mg protein (E1%/280)
mol wt
34.6 kDa
purified by
crystallization
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
storage temp.
−20°C
Application
A thermostable (thermophilic) extracellular metalloendopeptidase containing four calcium ions. Cofactors are zinc and calcium. Hydrolyzes protein bonds on the N-terminal side of hydrophobic amino acid residues. The pH optimum is 8.0 and the optimal temperature for activity is 70 °C. Considerably stable from pH 5 to 9.5. Thermolysin has a low cleavage specificity, therefore, it produces a number of short fragments that are suitable for sequencing. Preferential cleavage: X-cleavage-Y-Z where X=any amino acid; Y=Leu, Phe, Ile, Val, Met, Ala and Z is any amino acid other than Pro. Cleavage N-terminal to Leu is preferred over cleavage of N-terminal to Phe which is preferred over the others. Often used to do limited proteolysis for peptide mapping and studies of protein structure and conformational changes.
Physical form
lyophilized powder containing calcium and sodium acetate buffer salts
Other Notes
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
1 of 1
Questo articolo | ||
|---|---|---|
| description powder, BioReagent, 30-350 units/mg protein (E1%/280), suitable for cell culture | description Type X, lyophilized powder, 30-350 units/mg protein (E1%/280) | description lyophilized powder, ≥3,000 units/mg protein (biuret) |
| biological source Geobacillus stearothermophilus | biological source Geobacillus stearothermophilus | biological source - |
| specific activity 30-350 units/mg protein (E1%/280) | specific activity 30-350 units/mg protein (E1%/280) | specific activity ≥3,000 units/mg protein (biuret) |
| technique(s) single cell analysis: suitable, cell culture | mammalian: suitable | technique(s) - | technique(s) - |
| form powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 34.6 kDa | mol wt 34.6 kDa by amino acid sequence | mol wt - |
Still not finding the right product?
Explore all of our products under Thermolysin from Geobacillus stearothermophilus
Parola di avvertenza
Danger
Classe di stoccaggio
11 - Combustible Solids
Punto di infiammabilità (°F)
Not applicable
Punto di infiammabilità (°C)
Not applicable
DPI (dispositivi di protezione individuale)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Organi bersaglio
Respiratory system
Codici di pericolo
Avvertenze di sicurezza
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Che succede?
WGK 1
Scegli una delle versioni più recenti:
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
-
What are the molar extinction coefficient and molecular weight of this product?
1 risposta-
Utile?
-