SRP6369

AKR1D1 human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Sinonimo/i: 3o5bred, Aldo-keto reductase family 1, CBAS2, SRD5B1, member D1
• Codice UNSPSC: 12352200

Proprietà

• Origine biologica: human
• Ricombinante: expressed in E. coli
• Saggio: ≥90% (SDS-PAGE)
• Stato: liquid
• PM: 39.5 kDa (346 aa, 1-326 aa + His Tag)
• Confezionamento: pkg of 100 μg
• Concentrazione: 0.5 mg/mL
• N° accesso UniProt: P51857
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... AKR1D1(6718)

Descrizione

Descrizione generale

AKR1D1 (aldo-keto reductase 1D1) is the only steroid 5β-reductase in humans. This enzyme shows major expression in liver and resides in cytoplasm.[1] It is a member of the unique subfamily, AKR1D, of the aldo-keto reductase (AKR) superfamily, that are NAD(P)(H)-dependent oxidoreductases. AKR1D1 contains the characteristic (α/β)8 triosephosphate isomerase barrel in its core region, and has a molecular weight of 37kDa. Its C-terminal contains the active site residing on the β strands, and the ligand binding site is formed by long flexible loops.[2]
Recombinant human AKR1D1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Azioni biochim/fisiol

AKR1D1 (aldo-keto reductase 1D1) catalyzes the reduction of Δ⁴-3-ketosteroids to 5β-dihydrosteroids in a stereospecific manner, and is responsible for inducing A/B cis-configuration of bile acids. It is a critical enzyme for steroid hormone metabolism (except estrogens) and bile acid biosynthesis. Mutations in this gene are linked with bile acid deficiency resulting in usually fatal neonatal hepatitis and cholestasis.[1] Variants in this gene influence the expression of p450 enzymes, such as CYP3A4, CYP2C8, CYP2C9, CYP2C19, and CYP2B6, where up-regulation of AKR1D1 results in overexpression of these enzymes. Hence, this enzyme might indirectly be responsible for variation in drug metabolism or efficacy and adverse drug events in different individuals.[3]

Stato fisico

0.5 mg/mL in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 100 mM NaCl and 20% glycerol.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 13 - Non Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable