SRP0134

EZH2/EED/SUZ12/RbAp48/AEBP2 human

recombinant, expressed in baculovirus infected insect cells

• Sinonimo/i: ENX-1, EZH2, Enhancer of zeste homolog 2, KMT6A, Lysine N-methyltransferase 6A
• Codice UNSPSC: 12352200
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Ricombinante: expressed in baculovirus infected insect cells
• Saggio: ≥80% (SDS-PAGE)
• Stato: aqueous solution
• PM: 48 kDa (RbAp48); 51 kDa (EED); 53 kDa (AEBP2); 86 kDa (EZH2); 87 kDa (SUZ12)
• Confezionamento: pkg of 50 μg
• Concentrazione: >0.02 mg/mL
• N° accesso NCBI: NM_003797 (EED); NM_004456 (EZH2); NM_005610 (RbAP48); NM_015355 (SUZ12); NM_153207 (AEBP2)
• N° accesso UniProt: Q15910
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... EZH2(2146)

Descrizione

Descrizione generale

EZH2, EED, SUZ12, RbAp48 and AEBP2 are components of the polycomb repressive complex 2 (PRC2). The highly conserved complex methylates lysine 27 of histone H3. It also has a role in gene silencing and transcription repression.[1][2]

Applicazioni

EZH2/EED/SUZ12/RbAp48/AEBP2 human is useful for the study of enzyme kinetics,[3] screening inhibitors and selectivity profiling.

Definizione di unità

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

Stato fisico

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Nota sulla preparazione

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable