ELISA: 0.1-0.2 μg/mL using 2.5 μg/mL Human IgM myeloma for coating immunoblotting: suitable
Isotipo
IgG2b
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
Descrizione generale
Anti-Human IgM (m-chain specific) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the MB-11 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified human IgM protein.
Immunoglobulin M (IgM) is the major class of surface immunoglobulins on lymphocyte membranes. IgM is suggested to act as the first line of defense during microbial infections. IgM has a pentameric structure, in which monomers are linking together via disulfide bonds. Surface IgM is expressed on immature and mature B cells, whereas IgM heavy chain is expressed intracellularly in pre-B cells. IgM serves as the antigen receptor of naive B cells and is involved in B cell maturation and complement activation.
Specificità
Anti-Human IgM (m-chain specific) antibody, Mouse monoclonal specifically recognizes m chain of Human IgM. The antibody shows no cross-reactivity with human IgG, Fab, Fc, k, l, and g chains.
Immunogeno
Purified human IgM
Applicazioni
The antibody is recommended to use in various immunological techniques, including ELISA and Immunoblot.
Azioni biochim/fisiol
Immunoglobulin M (IgM) acts as an antigen specific part of the B cell antigen receptor on the surface of B lymphocytes that are not stimulated, in its monomeric form. Polymeric IgM molecules also serve as important activators of the classical complement cascade. IgM is essential in agglutination and cytolytic reactions. IgM serves as the antigen receptor of naive B cells and is involved in B cell maturation and complement activation.
Stato fisico
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.
Altre note
This product is for R&D use only, not for drug, household, or other uses.
Infection and immunity, 66(6), 2866-2870 (1998-05-29)
Streptococcus pneumoniae is a major respiratory pathogen of infants, children, and the elderly. Polysaccharide vaccines have been useful in adult populations but do not elicit protective immunity in infants and young children. To enhance their immunogenicity, vaccines of pneumococcal polysaccharides
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