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SAB4200114

Sigma-Aldrich

Anti-EDC4 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Sinonimo/i:

Anti-Ge-1, Anti-HEDLS (Human enhancer of decapping, (large subunit), Anti-RCD-8

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

rabbit

Coniugato

unconjugated

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Forma fisica

buffered aqueous solution

PM

antigen ~150 kDa

Reattività contro le specie

human

Concentrazione

~1.0 mg/mL

tecniche

immunoprecipitation (IP): 5-10 μg using ysates of HEK-293 cells over expressing human EDC4
indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed NIH-3T3 cells over expressing human EDC4
western blot: 0.5-1 μg/mL using lysates of HEK-293 cells over expressing human EDC4

N° accesso UniProt

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... EDC4(23644)
mouse ... Edc4(234699)
rat ... Edc4(361399)

Descrizione generale

Enhancer of mRNA-decapping protein 4 (EDC4), also known as human enhancer of decapping large subunit (HEDLS) or Ge-1, is characterized with an N-terminal WD40 repeat and a C-terminal domain which interacts with mRNA-decapping enzyme subunit 2 (Dcp2), and mediates EDC4 oligomerization and P-body localization .
The EDC4 gene maps on human chromosome 16q22.1.

Specificità

Anti-EDC4 (N-terminal) specifically recognizes human EDC4.

Applicazioni

Anti-EDC4 (N-terminal) antibody has been used in:
  • immunoprecipitation
  • immunofluorescence
  • immunoblotting.

Azioni biochim/fisiol

Human enhancer of mRNA-decapping protein 4 (EDC4) associates with hDcp2 and stimulates hDcp2 activity in vitro as well as mediating the interaction between mRNA-decapping enzyme subunit 1 & 2 (DCP1 and DCP2).
Interaction of processing-body components, EDC4 and Dcp1a is essential for the post-transcriptional regulation of interleukin (IL)-6.

Stato fisico

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stoccaggio e stabilità

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Target-specific requirements for enhancers of decapping in miRNA-mediated gene silencing
Eulalio A, et al.
Genes & Development, 21(20), 2558-2570 (2007)
Jucimara Colombo et al.
Oncology reports, 21(3), 649-663 (2009-02-13)
Laryngeal squamous cell carcinoma is very common in head and neck cancer, with high mortality rates and poor prognosis. In this study, we compared expression profiles of clinical samples from 13 larynx tumors and 10 non-neoplastic larynx tissues using a
Denis Chang et al.
The Journal of cell biology, 220(9) (2021-07-13)
Epithelial cells lining mucosal surfaces distinctively express the inflammatory bowel disease risk gene INAVA. We previously found that INAVA has dual and competing functions: one at lateral membranes where it affects mucosal barrier function and the other in the cytosol
The Assembly of EDC4 and Dcp1a into Processing Bodies Is Critical for the Translational Regulation of IL-6
Seto E, et al.
PLoS ONE, 10(5) (2015)
A human microprotein that interacts with the mRNA decapping complex
D'Lima N G, et al.
Nature Chemical Biology, 13(2), 174-174 (2017)

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