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Origine biologica
rabbit
Coniugato
peroxidase conjugate
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
secondary antibodies
Clone
polyclonal
Stato
lyophilized powder
Reattività contro le specie
chicken
tecniche
immunohistochemistry: suitable indirect ELISA: suitable western blot: suitable
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
modifica post-traduzionali bersaglio
unmodified
Descrizione generale
Chicken IgG (immunoglobulin G) is also referred to as IgY. IgY is an antibody produced in birds. IgY is similar to mammalian IgG but differs in some characteristics from IgG. IgY does not bind with mammalian complements nor rheumatoid factor, which are present in mammalian serum and anti-mammalian IgG antibodies. During oogenesis in chicken, IgY can pass across the follicular epithelium of the ovary and aggregate in the yolk. The heavy chain of IgY and mammalian IgG has a molecular weight of 68kDa and 50kDa, respectively. The structure of mammalian IgG is considered more stable than that of IgY. IgG antibodies, which often cross-react to IgG from different mammalian species, exhibit low cross-reactivity to IgY. Thus, IgY is beneficial when it is used as a biochemical/clinical reagent.
Specificità
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Peroxidase, Anti-Rabbit Serum, Chicken IgG, Chicken IgG F(ab′)2 and Chicken Serum. No reaction was observed against Chicken IgG F(c).
Immunogeno
Chicken IgG F(ab′)2 fragment
Proprietà fisiche
Antibody format: IgG
Stato fisico
Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Ricostituzione
Reconstitute with 1.0 mL deionized water (or equivalent).
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Production and purification of Fab? fragments from chicken egg yolk immunoglobulin Y (IgY)
Akita EM and Nakai S
Journal of Immunological Methods, 162(2), 155-164 (1993)
Molecular stability of chicken and rabbit immunoglobulin G.
Shimizu M
Bioscience, Biotechnology, and Biochemistry, 56(2), 270-274 (1992)
Immunoglobulins of the non-galliform birds: antibody expression and repertoire in the duck.
Lundqvist ML
Developmental and Comparative Immunology, 30(1-2), 93-100 (2006)
Domande
Recensioni
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