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S5394

Sigma-Aldrich

Lipoprotein Deficient Serum from fetal calf

frozen liquid

Sinonimo/i:

Fetal Calf Serum, Lipoprotein Deficient Serum

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.61

Origine biologica

bovine fetus

Livello qualitativo

200

Forma fisica

frozen liquid

Concentrazione

≥14 mg protein/mL Biuret

tecniche

cell culture | mammalian: suitable
cell culture | stem cell: suitable

Impurezze

<1 ng/mg protein endotoxin
<5 % of normal lipoprotein

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Categorie correlate

Descrizione generale

Studies show that the production of sterols is reduced in human fibroblasts cultured in the presence of lipoprotein deficient serum.

Applicazioni

Lipoprotein Deficient Serum from fetal calf has been used as a supplement in culture media to grow Chinese hamster ovary (CHO)-derived cells, thymic lobes and human peripheral blood mononuclear cells (PBMCs).

Nota sulla preparazione

Aseptically filtered, 0.2 μm
Dialyzed against Dulbecco′s phosphate buffered saline, pH 7.4-7.5

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves

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P M Giles et al.
Clinica chimica acta; international journal of clinical chemistry, 113(2), 183-191 (1981-06-18)
The biosynthesis and efflux of sterols from cells into the medium were investigated in skin fibroblasts from a control, a patient with obligate heterozygous familial hypercholesterolaemia and a patient with the homozygous condition. The behaviour of the cells was studied
Brian T Emmer et al.
PLoS genetics, 17(1), e1009285-e1009285 (2021-01-30)
Hypercholesterolemia is a causal and modifiable risk factor for atherosclerotic cardiovascular disease. A critical pathway regulating cholesterol homeostasis involves the receptor-mediated endocytosis of low-density lipoproteins into hepatocytes, mediated by the LDL receptor. We applied genome-scale CRISPR screening to query the
Lipid-mediated presentation of MHC class II molecules guides thymocytes to the CD4 lineage
Komaniwa S, et al.
European Journal of Immunology, 39(1), 96-112 (2009)
Sterol regulatory element-binding protein (SREBP) cleavage regulates Golgi-to-endoplasmic reticulum recycling of SREBP cleavage-activating protein (SCAP)
Shao W and Espenshade PJ
The Journal of Biological Chemistry, 289(11), 7547-7557 (2014)
Liliana M R Silva et al.
Frontiers in cellular and infection microbiology, 12, 809606-809606 (2022-03-01)
Apicomplexan parasites are considered as defective in cholesterol synthesis. Consequently, they need to scavenge cholesterol from the host cell by either enhancing the uptake of extracellular cholesterol sources or by upregulating host cellular de-novo biosynthesis. Given that Eimeria bovis macromeront
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