S5174
SPARC from murine parietal yolk sac (PYS-2) cells
~80% (SDS-PAGE), lyophilized powder
Sinonimo/i:
BM-40, Osteonectin, Secreted protein acidic and rich in cysteine
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About This Item
Origine biologica
mouse (PYS-2 cells)
Saggio
~80% (SDS-PAGE)
Stato
lyophilized powder
tecniche
cell culture | mammalian: suitable
Copertura della superficie
4‑40 μg/cm2
N° accesso UniProt
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
Informazioni sul gene
mouse ... Sparc(20692)
Applicazioni
SPARC has many useful properties including inhibiting cell spreading and diminishing focal contacts in vitro, abrogating the effects of PDGF and beta-FGF, binding directly to several components of the ECM, and undergoes proteolysis that exposes and releases regions in the protein that correlates with biological activities.
Azioni biochim/fisiol
SPARC is expressed in many tissues including bone, skin, skeletal muscle, reproductive tissues, and embryonic connective tissue. It is expressed in high levels in tissues that are undergoing morphogenesis, remodeling and repair.
Componenti
SPARC, or Secreted Protein Acidic and Rich in Cysteine, is a 283-287 AA Ca2+ binding to glycoprotein with an apparent molecular weight of 40-43 kDa.
Avvertenza
The lyophilized powder should be stored frozen at -20°C. The reconstituted product should be stored frozen at -70°C.
Nota sulla preparazione
This product is supplied as a lyophilized powder from phosphate buffered saline at 25 μg protein/vial. It should be reconstituted aseptically with sterile water, producing a solution of 0.1 to 0.3 mg protein per mL. SPARC is initially purified from mouse parietal yolk sac cells under conditions that preserve biological activity.
Codice della classe di stoccaggio
13 - Non Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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T F Lane et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 8(2), 163-173 (1994-02-01)
An extracellular matrix-associated glycoprotein expressed in a variety of tissues during embryogenesis and repair, SPARC contains modular domains that can function independently to bind cells and matrix components. Because SPARC can selectively disrupt cellular contacts with matrix and thereby effect
G Chen et al.
British journal of cancer, 102(1), 188-195 (2009-11-19)
Secreted protein acidic and rich in cysteine (SPARC) is a multi-faceted protein-modulating cell-cell and cell-matrix interactions. In cancer, SPARC can be not only associated with a highly aggressive phenotype, but also acts as a tumour suppressor. The aim of this
H Sage et al.
The Journal of cell biology, 109(1), 341-356 (1989-07-01)
SPARC (Secreted Protein Acidic and Rich in Cysteine) is a Ca+2-binding glycoprotein that is differentially associated with morphogenesis, remodeling, cellular migration, and proliferation. We show here that exogenous SPARC, added to cells in culture, was associated with profound changes in
Clément Faye et al.
The Journal of biological chemistry, 284(33), 22041-22047 (2009-06-23)
Endostatin is a C-terminal proteolytic fragment of collagen XVIII that is localized in vascular basement membrane zones in various organs. It binds to heparin/heparan sulfate and to a number of proteins, but its molecular mechanisms of action are not fully
Articoli
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