P7372

Anti-Protein Disulfide Isomerase (MD-12) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Sinonimo/i: Anti-Erp58, Anti-PDI
• Numero MDL: MFCD01094568
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Coniugato: unconjugated
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Forma fisica: buffered aqueous solution
• PM: antigen 57 kDa
• Reattività contro le specie: human, bovine, rat, mouse
• Convalida avanzata: independent; Learn more about Antibody Enhanced Validation
• tecniche: immunoprecipitation (IP): 2-5 μg using RIPA lysate (500 μg) of rat NRK cells; indirect immunofluorescence: 2-5 μg/mL using human HeLa cells; western blot (chemiluminescent): 0.1-0.2 μg/mL using whole extract of mouse NIH3T3 cells
• N° accesso UniProt: P07237
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... P4HB(5034) ; mouse ... P4hb(18453) ; rat ... P4hb(25506)

Descrizione

Descrizione generale

Anti-Protein Disulfide Isomerase (PDI) (MD-12) is developed in rabbit using as immunogen a synthetic peptide corresponding to amino acid residues of human PDI with N-terminal added cysteine, conjugated to KLH. Protein disulfide isomerase (PDI, Erp58) is an abundant multifunctional, soluble enzyme (E.C. 5.3.4.1) that resides in the lumen of the endoplasmic reticulum of eukaryotic cells. The mammalian PDI family comprises several highly divergent proteins that contain one or more thioredoxin like structural domains. PDI consists of four tandem domains, two of which contain a catalytic site for S-S bond formation. PDI has an N-terminal ER signal and C-terminal ER retention KDEL signal sequences. PDI may also be expressed in other cellular localizations such as the cell surface, cytosol, and nucleus. PDI was found on the cell surface of several cell types including platelets, lymphoid cells, pancreatic exocrine cells, retinal cells, thyroid cells, and hepatocytes.

Immunogeno

synthetic peptide corresponding to amino acid residues 495-506 of human protein disulfide isomerase.

Applicazioni

Anti-Protein Disulfide Isomerase (MD-12) antibody produced in rabbit has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Azioni biochim/fisiol

Protein disulfide isomerase (PDI) catalyses the formation and rearrangements of both intrachain and interchain disulfide bonds in secreted proteins. PDI also serves as a molecular chaperone that can suppress protein aggregation, or as an anti-chaperone that mediates aggregate formation. PDI respectively participates in the hydroxylation of prolines in procollagen during collagen synthesis and in the transfer of neutral lipid onto nascent lipoprotein particles. PDI has calcium-dependent transglutaminase activity, which catalyses the formation of isopeptide bonds.

Stato fisico

Solution in 0.01 M phosphate buffered saline containing 1% bovine serum abumin and 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable