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MAK206

Sigma-Aldrich

Fumarase Activity Colorimetric Assay Kit

Sufficient for 100 Colorimetric tests

Sinonimo/i:

Fumarate Hydratase Activity Colorimetric Assay Kit

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
12352200
NACRES:
NA.84

Metodo di rivelazione

colorimetric

Malattie correlate

neurological disorders

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... FH(2271)
mouse ... FH(14193)
rat ... FH(24368)

Descrizione generale

The new Fumarase Assay Kit, MAK521, is now available! Fumarase (Fumarate Hydratase; EC 4.2.1.2) catalyzes the reversible hydration of fumarate to malate in the tricarboxylic acid (Krebs) cycle. The activity of fumarase is important as it produces reducing equivalents that drive the synthesis of ATP and maintains energy balance in aerobic metabolism. A deficiency of fumarase activity results in progressive encephalopathy.

Applicazioni

Fumarase Activity Colorimetric Assay Kit has been used to determine fumarase (FUM) activity.

Compatibilità

Suitable for the detection of Fumarase in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.

Principio

The Fumarase Activity Colorimetric Assay Kit provides a simple and high-throughput ready procedure for measuring fumarase activity in a variety of tissues, cells, and isolated mitochondria. Fumarase activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of fumarase is the amount of enzyme that generates 1.0 mmole of NADH per minute at pH 9.5 at 37 °C.

Solo come componenti del kit

N° Catalogo
Descrizione

  • Fumarase Assay Buffer

  • Fumarase Substrate

  • Fumarase Enzyme Mix

  • Fumarase Developer

  • NADH Standard

  • Fumarase Positive Control

Sostituito da

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Corrosion

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Eye Dam. 1 - Skin Corr. 1B

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials


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Acute Lung Injury (ALI), characterized by bilateral pulmonary infiltrates that restrict gas exchange, leads to respiratory failure. It is caused by an innate immune response with white blood cell infiltration of the lungs, release of cytokines, an increase in reactive
Ping Li et al.
International journal of molecular sciences, 23(3) (2022-02-16)
The FH gene encodes the fumarate hydratase of the Krebs cycle and functions as a homotetramer to catalyze the hydration of fumarate to malate. Mutations in FH result in uterine leiomyomas, a rare autosomal dominant inherited metabolic disease. However, how

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