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MAK056

Sigma-Aldrich

Choline/Acetylcholine Quantification Kit

sufficient for 100 colorimetric or fluorometric tests (for choline assay and 50 tests acetylcholine assay)

Sinonimo/i:

Choline/Acetylcholine Assay Kit

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About This Item

Codice UNSPSC:
12161503
NACRES:
NA.25

impiego

sufficient for 100 colorimetric or fluorometric tests (for choline assay and 50 tests acetylcholine assay)

Metodo di rivelazione

colorimetric
fluorometric

Temperatura di conservazione

−20°C

Descrizione generale

Choline is an essential nutrient, commonly grouped with the B complex vitamins, that plays key roles in many biological processes. Choline is a precursor for the synthesis of acetylcholine, a critical neurotransmitter in both the peripheral and central nervous systems.

Applicazioni

Choline/Acetylcholine Quantification Kit has been used to quantify the amounts of choline and acetylcholine in samples.

Compatibilità

Suitable for use with cell and tissue culture supernatants as well as serum, plasma, urine, and other biological fluids.

Principio

In this assay, choline concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the choline present. Acetylcholine levels can be determined by adding acetylcholinesterase to the reaction, which hydrolyzes acetylcholine to choline and acetate.

Sostituito da

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Pittogrammi

Health hazardExclamation mark

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

188.6 °F - closed cup

Punto d’infiammabilità (°C)

87 °C - closed cup


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