M0321

MDM2

recombinant, expressed in E. coli, buffered aqueous glycerol solution, ≥75% (SDS-PAGE)

• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Origine biologica: human
• Ricombinante: expressed in E. coli
• Descrizione: contains an N-terminal GST tag
• Saggio: ≥75% (SDS-PAGE)
• Stato: buffered aqueous glycerol solution
• PM: ~84 kDa
• N° accesso UniProt: Q00987
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... MDM2(4193)

Descrizione

Descrizione generale

Murine double minute 2 (MDM2) encoding gene is localized at 12q15 in the human chromosome.[1] It is a multidomain nuclear phosphoprotein.[2] It possesses really interesting new gene (RING) finger domain and its function and structural integrity is required for the action of MDM2. It also has central acidic domain.[3]

Azioni biochim/fisiol

MDM2 is a negative regulator of the p53 tumor suppressor.

Mdm2 functions as an E3 ubiquitin ligase and an inhibitor of p53 transcriptional activation.

Murine double minute 2 (MDM2) is a chief regulator of the p53 tumor suppressor protein.[4] It is a multifunctional oncoprotein that contributes to both p53-dependent and -independent roles in oncogenesis. MDM2 has various p53-independent functions that contribute to its activity as an oncogene.[5]

Overexpression of Murine double minute 2 (MDM2) is observed in various cancer like lung, gastric, colorectal, hepatocellular, breast, endometrial, and prostate. A common single nucleotide polymorphism is observed at 309 amino acid (from T to G) increases the susceptibility to cancer.[6]

Stato fisico

Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF and 25% glycerol.

Nota sulla preparazione

Supplied as a truncated version (202-end) with an N-terminal GST tag

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)