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L3759

Sigma-Aldrich

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

biotin conjugate, lyophilized powder

Sinonimo/i:

Bandeirea simplicifolia agglutinin, BS-I

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About This Item

Codice UNSPSC:
12352200
NACRES:
NA.32

Coniugato

biotin conjugate

Forma fisica

lyophilized powder

Potenza

<250 μg per mL agglutination activity (using human blood group A erythrocytes.)

Composizione

Protein, ~85% E1%/280

Grado di funzionalizzazione

~5 mol biotin per mol protein

Temperatura di conservazione

2-8°C

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Applicazioni

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) has been used:
  • for highlighting murine endothelial cells
  • to identify fetal capillaries in endothelial immunohistochemical staining, conjugated with biotin
  • to detect tumor total vessels

Azioni biochim/fisiol

BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Altre note

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Stato fisico

Contains sodium citrate

Risultati analitici

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

Codice della classe di stoccaggio

13 - Non Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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