KSPQ12012
KiCqStart® SYBR® Green Primers
Predesigned primers for gene expression analysis
Sinonimo/i:
Pre-designed RT-qPCR Primers, SYBR Green RT-qPCR Primers
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Ordering predesigned KiCqStart SYBR® Green primers for your gene targets is easy using Sigma's state-of-the-art oligonucleotide configuration tool. Open the tool, enter your gene, select your species, choose your primer sets, and add to cart.
Prodotti consigliati
Temperatura di conservazione
−20°C
Descrizione generale
Overview
Compatible with any thermal cycler, KiCqStart Primers are ready-to-order, pre-designed primer pairs for quantifying gene expression by SYBR Green I RT-qPCR (Reverse Transcription Quantitative Real-Time PCR). To comply with MIQE, they have been developed using sophisticated bioinformatics tools and validated in silico to avoid off-target amplification1. KiCqStart Primers are available as up to three ranked sets of forward and reverse primer pairs for all genes from common model organisms.
1SNP loci have been avoided during the design process when possible. Please consult dbSNP to determine if your gene has unavoidable SNPs.
To learn more about KiCqStart Primers visit our Frequently Asked Questions section.
Specifications
Design characteristics
Oligo characteristics
Sequences are provided at the time of shipment.
295% of primer pairs fall within these specifications
Rank
Each gene has up to three alternative primer pairs, which are identified as follows:
Compatible with any thermal cycler, KiCqStart Primers are ready-to-order, pre-designed primer pairs for quantifying gene expression by SYBR Green I RT-qPCR (Reverse Transcription Quantitative Real-Time PCR). To comply with MIQE, they have been developed using sophisticated bioinformatics tools and validated in silico to avoid off-target amplification1. KiCqStart Primers are available as up to three ranked sets of forward and reverse primer pairs for all genes from common model organisms.
1SNP loci have been avoided during the design process when possible. Please consult dbSNP to determine if your gene has unavoidable SNPs.
To learn more about KiCqStart Primers visit our Frequently Asked Questions section.
Specifications
Design characteristics
- GC Content: 20 - 80%2
- Melting Temperature: 55.0 ± 5 ºC
- Secondary Structure: None
- Excessive 3′ Clamping: Minimal
- Homology: None
Oligo characteristics
- Molecule: DNA (all sequences verified by mass spec QC); no modifications
- Contents: 1 forward and 1 reverse primer
- Length: 18 - 24 bases2
- Purification: RP cartridge
- Format: 3 OD minimum yield, dry; 1 oligonucleotide per tube
Sequences are provided at the time of shipment.
295% of primer pairs fall within these specifications
Rank
Each gene has up to three alternative primer pairs, which are identified as follows:
- 1: The middle of one primer overlaps an exon splice site
- 2: The 5′ or 3′ end of one primer overlaps an exon splice site
- 3: The amplicon, but neither primer, overlaps an exon splice site
- 4: Primer overlap is not possible for various reasons, e.g. the gene has only one exon (primer pairs with this rank may lead to co-amplification of genomic DNA).
Applicazioni
Product use: gene expression analysis
Caratteristiche e vantaggi
KiCqStart Primers are sensitive, specific, and will save you time and money by eliminating the tedious steps required to develop an effective SYBR Green I RT-qPCR Assay.
Benefits include:
Benefits include:
- Target analysis is done
- Design characteristics are set
- Oligo characteristics are set
- Easy to order
Proprietà fisiche
Properties include:
- Available species: human, mouse, rat, zebrafish, cow, horse, Arabidopsis, chicken, dog, rabbit, pig, cat, rice, soybean, rhesus, Drosophila, C. elegans, corn, X. laevis, Guinea Pig, and Chinese Hamster.
- Components: two primers, one oligo per tube
- Shipping condition: dry, ambient temperature
- Storage condition: dry or wet single-use aliquots
Altre note
Frequently Asked Questions section is available for this product.
Note legali
KiCqStart is a registered trademark of QIAGEN Beverly Inc.
SYBR is a registered trademark of Life Technologies
Raccomandato
N° Catalogo
Descrizione
Determinazione del prezzo
Scegli una delle versioni più recenti:
Certificati d'analisi (COA)
Lot/Batch Number
Ci dispiace, ma al momento non ci sono COA disponibili online per questo prodotto.
Se ti serve aiuto, non esitare a contattarci Servizio Clienti
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
Aaminah Khan et al.
Nature communications, 12(1), 971-971 (2021-02-14)
Diffuse intrinsic pontine glioma (DIPG) is an incurable malignant childhood brain tumor, with no active systemic therapies and a 5-year survival of less than 1%. Polyamines are small organic polycations that are essential for DNA replication, translation and cell proliferation.
Ana Paula Oliveira De Quadros et al.
Journal of toxicology and environmental health. Part A, 83(13-14), 495-508 (2020-06-23)
Sm. (Rosaceae) is a plant traditionally used in Brazil and some other countries to treat diarrhea, stomach diseases, and as an analgesic, antimicrobial, antihypertensive, and as well as other pharmacological properties. The aim of this study was to examine cytotoxic
Agata Nyga et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 107(5), 1352-1362 (2018-09-28)
We aimed to find a biomarker for patients with adverse reaction to metal debris (ARMD) due to a metal-on-metal (MoM) hip implant. First, we compared molecular markers of hypoxia-inducible factor (HIF) pathway activation (BNIP3, GLUT1, HO1, VEGF, and HIF1A) and
Mara Ribeiro Almeida et al.
Molecular nutrition & food research, 60(7), 1615-1624 (2016-03-05)
Vitamin B6 plays crucial roles on brain development and its maternal deficiency impacts the gamma-aminobutyric acid (GABA)ergic, serotonergic, glutamatergic, and dopaminergic systems in offspring. However, the molecular mechanisms underlying these neurological changes are not well understood. Thus, we aimed at
Helen Heath et al.
The EMBO journal, 27(21), 2839-2850 (2008-10-17)
The 11-zinc finger protein CCCTC-binding factor (CTCF) is a highly conserved protein, involved in imprinting, long-range chromatin interactions and transcription. To investigate its function in vivo, we generated mice with a conditional Ctcf knockout allele. Consistent with a previous report
Articoli
Primer pairs detect prevalent eukaryotic genes, but may co-amplify other transcripts, emphasizing the importance of comprehensive primer design.
Filtri attivi
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.
