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I5283

Sigma-Aldrich

Anti-INCENP antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Sinonimo/i:

Anti-Inner Centromere Protein

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About This Item

Numero MDL:
MFCD05665235
Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

rabbit

Coniugato

unconjugated

Forma dell’anticorpo

IgG fraction of antiserum

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Forma fisica

buffered aqueous solution

PM

antigen ~106 kDa

Reattività contro le specie

human, mouse, rat

tecniche

indirect immunofluorescence: 1:100 using HeLa cells
microarray: suitable
western blot (chemiluminescent): 1:2,000 using whole extract of mouse NIH3T3 and rat PC12 cells
western blot (chemiluminescent): 1:5,000 using nuclear extract of human HeLa cells

N° accesso UniProt

Q9NQS7

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... INCENP(3619)
mouse ... Incenp(16319)
rat ... Incenp(293733)

Descrizione generale

The gene INCENP (inner centromere protein) encodes an inner centromere protein that forms a component of the chromosomal passenger complex (CPC). It is found to be localized to inner centromeres during mitosis. The CPC also contains Aurora B, survivin, and Borealin.

Immunogeno

synthetic peptide corresponding to amino acid residues 884-901 of human INCENP with N-terminal added cysteine conjugated to KLH. The corresponding sequence differs by one amino acid in chicken and Xenopus laevis and by two amino acids in mouse.

Applicazioni

Anti-INCENP antibody produced in rabbit has been used in western blotting and immunofluorescence.

Azioni biochim/fisiol

Studies in knockout mice established INCENP (inner centromere protein) as essential for mouse development and viability. Loss of INCENP results in chromosome segregation defects and failures in cytokinesis.
The inner centromere protein encoded by the INCENP gene is phosphorylated by Cdk1 (cyclin dependent kinase 1) in mitosis. It is found to be associated with Plk1 (Polo-like kinase) in a polo-box–dependent manner. Plk1 is a serine/threonine kinase that functions in cell cycle, especially in mitosis. INCEP participates in the localization of Bub1 (Budding uninhibited by benzimidazole 1), a serine/threonine kinase involved in spindle checkpoint, to kinetochore. This, in turn, promotes the recruitment of Plk1 to kinetochores. Phosphorylated INCENP stimulates the activity of Aurora B kinase at centromeres and the central spindle that is important for chromosome segregation and cytokinesis, respectively.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificati d'analisi (COA)

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A Cul3-based E3 ligase removes Aurora B from mitotic chromosomes, regulating mitotic progression and completion of cytokinesis in human cells
Sumara I, et al.
Developmental Cell, 12(6), 887-900 (2007)
Lin-Ing Wang et al.
PLoS genetics, 15(5), e1008072-e1008072 (2019-06-01)
Sister centromere fusion is a process unique to meiosis that promotes co-orientation of the sister kinetochores, ensuring they attach to microtubules from the same pole during metaphase I. We have found that the kinetochore protein SPC105R/KNL1 and Protein Phosphatase 1
Reiko Honda et al.
Molecular biology of the cell, 14(8), 3325-3341 (2003-08-20)
The function of the Aurora B kinase at centromeres and the central spindle is crucial for chromosome segregation and cytokinesis, respectively. Herein, we have investigated the regulation of human Aurora B by its complex partners inner centromere protein (INCENP) and
Renee J Tamming et al.
Cell reports, 31(13), 107838-107838 (2020-07-02)
ATRX gene mutations have been identified in syndromic and non-syndromic intellectual disabilities in humans. ATRX is known to maintain genomic stability in neuroprogenitor cells, but its function in differentiated neurons and memory processes remains largely unresolved. Here, we show that
Nootan Pandey et al.
The Journal of cell biology, 219(1) (2019-11-13)
Topoisomerase II (Topo II) is essential for mitosis since it resolves sister chromatid catenations. Topo II dysfunction promotes aneuploidy and drives cancer. To protect from aneuploidy, cells possess mechanisms to delay anaphase onset when Topo II is perturbed, providing additional
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