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Merck
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HLP501

Sigma-Aldrich

Normal Human Intrahepatic Biliary Epithelial Cells (Cholangioctyes); passage 1

Single-Donor Cryopreserved Cholangioctyes cells isolated from human livers, high viability, and characterized by cell specific markers

Sinonimo/i:

Homogenous population isolated from whole human livers

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About This Item

Codice UNSPSC:
41106509
NACRES:
NA.81

Origine biologica

human liver

Forma fisica

frozen liquid

Confezionamento

pkg of 1 vial (contains ≥0.25 million cells)
pkg of 1 vial (contains ≥0.5 million cells)
pkg of 1 vial (contains ≥1 million cells)

tecniche

cell culture | mammalian: suitable

Colore

cloudy

Temperatura di conservazione

-140 to -196°C

Descrizione generale

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Primary human Cholangiocytes, also known as human intrahepatic biliary epithelial cells, are epithelial cells that line the intrahepatic bile ducts. Chonlangiocytes are important for ductal bile modification and are targeted in multiple liver diseases, such as primary biliary cirrhosis, cholangiocarcinoma and sclerosing cholangitis.

Applicazioni

Human cholangiocytes are ideal in studies of: toxicity, bile production and absorption, bile duct fibrosis, hormone signaling, cholangitis, cholangiocarcinoma and more. We offer matched sets of cholangiocytes cells from same donor as Hepatocytes, which can be used for co-culture systems to better simulate the complex microenvironment of the liver and gain insights into various liver-related diseases and conditions.

Caratteristiche e vantaggi

Our cryopreserved human Cholangiocytes cells are derived from the human liver, with each donor providing documented consent for research use of non-transplantable organs or tissues. Following the primary culture, these cells are cryopreserved. Each lot undergoes testing for specific cell markers and comes with a guarantee of ≥ 70% post-thaw viability. In addition, all cells have been tested for the absence of HIV, HBV, HCV, Syphillis and microbial contaminants (fungi, bacteria, and mycoplasma).

Attenzione

Each donor is tested and found non-reactive to viral DNA from HIV-1, HIV-2, HTLV I, HTLV II, hepatitis B and hepatitis C. However, no known test can offer complete assurance that the viruses that cause HIV-1, HIV-2, HTLV I, HTLV II, hepatitis B and hepatitis C are not present. Proper precautions and biological containment should be taken when handling cells of human origin, due to their potential biohazardous nature. All human based products should be handled at a BSL-2 (Biosafety Level 2) or higher.

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

Explore answers to frequently asked questions (FAQs) about culturing primary human hepatocytes and other liver cells including Stellate cells, Kupffer cells, Sinusoidal Endothelial cells, and more.

Protocolli

Discover our collection of primary human hepatic Kupffer cells and protocol for thawing, plating, and growing Kupffer cells. Find the formulation for Kupffer culture maintenance media.

Discover our collection of primary human hepatic sinusoidal endothelial cells, a protocol for thawing, plating, and growing HHSECs, and a recipe for our HHSEC maintenance media formulation.

Contenuto correlato

Explore our primary liver cells collection, including primary human Hepatocytes (PHH), spheroid-qualified Hepatocytes, Kupffer cells, Stellate cells, Cholangiocytes, and Sinusoidal Endothelial cells.

Discover our collection of primary human hepatic stellate cells and protocol for thawing, plating, and growing stellate cells. Find our stellate culture maintenance media formulation.

Discover our primary human intrahepatic biliary epithelial cells, also known as cholangiocytes. Explore our plating and maintenance media formulations and protocol for culture.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.