G4134

Glucosio-6-fosfato deidrogenasi

Type IX, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)

• Sinonimo/i: G-6-P-DH, Zwischenferment
• Numero CAS: 9001-40-5
• Classificazione EC (Enzyme Commission): 1.1.1.49 (BRENDA, IUBMB)
• Numero CE: 232-602-6
• Numero MDL: MFCD00081656
• Codice UNSPSC: 12352204
• NACRES: NA.54

Proprietà

• Tipo: Type IX
• Livello qualitativo: 200
• Stato: lyophilized powder
• Attività specifica: 200-400 units/mg protein (modified Warburg-Christian)
• PM: 128 kDa
• Tenore di β-NADP e β-NADPH: ≤10 mmol/mol
• applicazioni: agriculture
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Research area: Cell Signaling

Glucose-6-phosphate dehydrogenase (G6PD) is a key metabolic enzyme of the pentose phosphate pathway. In S. cerevisiae, it is encoded by the ZWF1 gene. G6PD exists as a tetramer in its active form.[1]

Applicazioni

Glucose-6-phosphate dehydrogenase is used:

• To test ketose reductase activity in developing maize endosperm.[2]
• For recycling microassay of β-NADP and β-NADPH.[3][4]
• To measure the intracellular levels of NADPH and total NADP.[5]
• To measure the nicotinamide adenine dinucleotide (NAD) kinase kinetic assay activity.[6]

Azioni biochim/fisiol

La glucosio-6-fosfato deidrogenasi catalizza la conversione del glucosio-6-fosfato in 6-fosfoglucono-acetone come prima fase della via dei pentoso fosfati.

Glucose-6-phosphate dehydrogenase catalyzes the rate-limiting step in the pentose phosphate pathway. Its function involves the conversion of glucose-6-phosphate to 6-phosphogluconolacetone while generating NADPH, which is essential for the regeneration of glutathione The glutathione system utilizes nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) to effectively eliminate excess hydrogen peroxide.[7] Glucose-6-phosphate dehydrogenase (G6PD) plays an important role in regulating cell growth and survival. Their levels are higher in cells undergoing normal and neoplastic growth. Increased glucose-6-phosphate dehydrogenase activity plays a pivotal role in preventing reactive oxygen species mediated cell death.[8] Glucose-6-phosphate dehydrogenase is over expressed in several cancers whereas its activity is reduced in hyperglycemia. A deficiency in glucose-6-phosphate dehydrogenase causes hemolysis.[9]

Definizione di unità

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.

Stato fisico

Lyophilized powder essentially sulfate-free, containing approx. 20% sodium citrate

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H334
• Consigli di prudenza: P261 - P284 - P501
• Classi di pericolo: Resp. Sens. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)