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G3795

Anti-Gravin antibody, Mouse monoclonal

Name: Anti-Gravin antibody, Mouse monoclonal
Brand: SIGMA

clone JP74, purified from hybridoma cell culture

Sinonimo/i:

Anti-A-Kinase Anchor Protein 12, Anti-AKAP12

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About This Item

Numero MDL:

MFCD08705585

Codice UNSPSC:

12352203

NACRES:

NA.44

Origine biologica

mouse

Coniugato

unconjugated

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

JP74, monoclonal

Forma fisica

buffered aqueous solution

Reattività contro le specie

mouse, rat, human

tecniche

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.02-0.04 μg/mL using rat brain cytosolic S1 fraction

Isotipo

IgG2a

N° accesso UniProt

Q02952

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... AKAP12(9590)
mouse ... Akap12(83397)

Descrizione generale

Monoclonal Anti-Gravin (mouse IgG2a isotype) is derived from the hybridoma JP74 produced by the fusion of mouse myeloma cells (X63Ag8.653) and splenocytes from BALB/c mice immunized with fractionated membrane cortex proteins from human neurons [cell lines NT2-N and SK-N-BE(2)]. Gravin protein is a myristoylated high molecular weight cytoplasmic protein expressed in endothelial cells, cultured fibroblasts and osteosarcoma cells and highly expressed in the brain.

Specificità

Monoclonal Anti-Gravin antibody is specific for human, rat and mouse gravin (approx. 300 kDa).

Immunogeno

fractionated membrane cortex proteins from human neuron cell lines NT2-N and SK-N-BE(2).

Applicazioni

Anti-Gravin antibody has been used in western blotting immunofluorescence.

Monoclonal Anti-Gravin antibody is suitable for use in immunoblotting (using mpkCCD cells ). The product may also be used for immunocytochemistry and immunoprecipitation.

Azioni biochim/fisiol

Gravin (or AKAP12) is a scaffolding kinase that functions as a tumor suppressor. This kinase interacts with PKC, PKA and other cell cycle signaling proteins to modulate cell division, growth, differentiation and inflammatory responses. The expression of gravin isoforms (α, β, and γ) is regulated by tissue-specific promoters. Gravin also acts as an autoantigen in myasthenia gravis patients .

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificati d'analisi (COA)

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Multiple promoters direct expression of three AKAP12 isoforms with distinct subcellular and tissue distribution profiles.

Jeffrey W Streb et al.

The Journal of biological chemistry, 279(53), 56014-56023 (2004-10-22)

A Kinase Anchoring Protein 12 (AKAP12; also known as src-suppressed C kinase substrate (SSeCKS) and Gravin) is a multivalent anchoring protein with tumor suppressor activity. Although expression of AKAP12 has been examined in a number of contexts, its expression control

The role of SSeCKS/gravin/AKAP12 scaffolding proteins in the spaciotemporal control of signaling pathways in oncogenesis and development .

Irwin H Gelman

Frontiers in bioscience : a journal and virtual library, 7, d1782-d1797 (2002-07-23)

Scaffolding proteins are thought to facilitate the efficiency and specificity of enzyme/substrate reactions by coordinating their interaction along a cytoskeletal infrastructure in a spacial and temporal manner. Rodent SSeCKS, and its human orthologue, Gravin, seem to function as tumor suppressors

Gravin regulates centrosome function through PLK1

Colicino E G, et al.

Molecular Biology of the Cell, 29(5), 532-541 (2018)

Quantitative protein and mRNA profiling shows selective post-transcriptional control of protein expression by vasopressin in kidney cells.

Sookkasem Khositseth et al.

Molecular & cellular proteomics : MCP, 10(1), M110-M110 (2010-10-14)

Previous studies in yeast have supported the view that post-transcriptional regulation of protein abundances may be more important than previously believed. Here we ask the question: "In a physiological regulatory process (the response of mammalian kidney cells to the hormone

Gravin, an autoantigen recognized by serum from myasthenia gravis patients, is a kinase scaffold protein.

J B Nauert et al.

Current biology : CB, 7(1), 52-62 (1997-01-01)

Subcellular targeting of protein kinases and phosphatases provides a mechanism for co-localizing these enzymes with their preferred substrates. A recently identified mammalian scaffold protein, AKAP79, controls the location of two broad-specificity kinases and a phosphatase. We have identified and characterized

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