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F7053

Sigma-Aldrich

Anti-vinculina monoclonale

clone hVIN-1, purified from hybridoma cell culture

Sinonimo/i:

Monoclonal Anti-Vinculin antibody produced in mouse

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About This Item

Numero MDL:
MFCD00162905
Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

mouse

Coniugato

FITC conjugate

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

hVIN-1, monoclonal

Forma fisica

buffered aqueous solution

PM

antigen 116 kDa

Reattività contro le specie

frog, chicken, mouse, canine, human, bovine, rat, turkey

Condizioni di stoccaggio

protect from light

tecniche

direct immunofluorescence: 1:50 using methanol/acetone fixed cultured bovine MDBK cells

Isotipo

IgG1

N° accesso UniProt

P18206

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)

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Specificità

Marca specificamente la vinculina in corrispondenza dei contatti cellula-cellula e cellula-substrato. Reagisce fortemente con la vinculina umana. Mostra reattività crociata con la metavinculina dei muscoli lisci.

Immunogeno

vinculina umana purificata da utero.

Applicazioni

Monoclonal Anti-Vinculin-FITC antibody is suitable for immunofluorescence staining of fibroblasts cells to study the effects of nanofiber spatial organization on cell behaviors. It is also suitable for direct immunofluorescence at a dilution of 1:50 using methanol/acetone fixed cultured bovine MDBK cells.

Azioni biochim/fisiol

Vinculin is a cytoskeletal protein with molecular mass of ~116 kDa. Vinculin is closely associated with adhesive membrane areas, such as focal contacts, podosomes, or intercellular junctions of the zonula adherens type. It acts as a mediator in interactions of integrins and the actin cytoskeleton. It forms a bridge between integrins, receptors for ECM molecules on the cell surface and the F-actin cytoskeleton. It is associated with talin and α-actinin which directly connects with integrins. It has ability to bind α-catenin as well as the vasodilator stimulated phosphoprotein (VASP) in focal contact assembly.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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E E Weiss et al.
The Journal of cell biology, 141(3), 755-764 (1998-06-13)
In epithelial cells, alpha-, beta-, and gamma-catenin are involved in linking the peripheral microfilament belt to the transmembrane protein E-cadherin. alpha-Catenin exhibits sequence homologies over three regions to vinculin, another adherens junction protein. While vinculin is found in cell-matrix and
Leonel E Rojo et al.
Journal of cosmetic dermatology, 9(3), 185-195 (2010-10-05)
Cell migration, angiogenesis, inflammation, and extracellular matrix remodeling are key events in wound healing. Natural products, including fatty acids (FAs), can accelerate wound healing by modulating the aforementioned events. This study aims to evaluate the effect of lucuma (Pouteria lucuma
Carol Jurchenko et al.
Biophysical journal, 106(7), 1436-1446 (2014-04-08)
The interplay between chemical and mechanical signals plays an important role in cell biology, and integrin receptors are the primary molecules involved in sensing and transducing external mechanical cues. We used integrin-specific probes in molecular tension fluorescence microscopy to investigate
A Westmeyer et al.
The EMBO journal, 9(7), 2071-2078 (1990-07-01)
We have analyzed the functional domain structure of vinculin, a protein involved in linking microfilaments to the cytoplasmic face of cell membranes in animal cells. For this purpose, we used several monoclonal antibodies raised against chicken gizzard vinculin whose epitopes
Mathieu Maalouf et al.
Nanomaterials (Basel, Switzerland), 12(10) (2022-05-29)
Ultrashort pulse lasers have significant advantages over conventional continuous wave and long pulse lasers for the texturing of metallic surfaces, especially for nanoscale surface structure patterning. Furthermore, ultrafast laser beam polarization allows for the precise control of the spatial alignment
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