Various solvents, including ethanol, are reported to inhibit forskolin-mediated activation of adenylate cyclase. Dimethyl sulfoxide (DMSO) is the recommended solvent; at a final concentration of 5% or less, little if any inhibition of adenylate cyclase activation will occur. A 50 mM stock solution of forskolin can be prepared by solubilizing 10 mg of forskolin in 488 µL of fresh, high purity, and anhydrous DMSO. After reconstitution, aliquot and freeze at -20°C. The 50 mM stock solution can be diluted 1:100 in DMSO to achieve a 500 µM working stock solution.
F3917
Forskolina
For use in molecular biology applications
Sinonimo/i:
7β-acetossi-8,13-epossi-1α,6β,9α-triidrossilabd-14-en-11-one, Coleonolo, Colforsina
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Cambia visualizzazione
| Voi/SKU | Disponibilità | Prezzo |
|---|---|---|
10 mg | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 324,00 € |
25 mg | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 701,00 € |
Informazioni su questo articolo
Formula empirica (notazione di Hill):
C22H34O7
Numero CAS:
Peso molecolare:
410.50
UNSPSC Code:
12352200
NACRES:
NA.52
PubChem Substance ID:
EC Number:
266-410-9
Beilstein/REAXYS Number:
1692716
MDL number:
Assay:
≥98% (HPLC)
Form:
powder
324,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Servizio Tecnico
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Permettici di aiutartibiological source
Coleus forskohlii
Quality Segment
description
for activation of T cells
product line
BioReagent
assay
≥98% (HPLC)
form
powder
mol wt
410.50 g/mol
color
white to off-white
solubility
ethanol: soluble
suitability
suitable for inhibits interleukin 2 (IL-2) production in Jurkat cells
storage temp.
room temp
SMILES string
CC(=O)O[C@H]1[C@@H](O)[C@H]2C(C)(C)CC[C@H](O)[C@]2(C)[C@@]3(O)C(=O)C[C@@](C)(O[C@]13C)C=C
InChI
1S/C22H34O7/c1-8-19(5)11-14(25)22(27)20(6)13(24)9-10-18(3,4)16(20)15(26)17(28-12(2)23)21(22,7)29-19/h8,13,15-17,24,26-27H,1,9-11H2,2-7H3/t13-,15-,16-,17-,19-,20-,21+,22-/m0/s1
InChI key
OHCQJHSOBUTRHG-KGGHGJDLSA-N
General description
La forskolina è un diterpenoide permeabile alle cellule isolato da Coleus forskohlii.
Biochem/physiol Actions
Diterpenoide permeabile a livello cellulare con proprietà antipertensiva, inotropa positiva e di attivazione dell′adenilciclasi. Molti dei suoi effetti biologici sono dovuti all′attivazione dell′adenilciclasi e all′incremento della concentrazione intracellulare dell′cAMP che ne consegue. La forskolina induce correnti di calcio e inibisce la MAP chinasi.
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Questo articolo | |||
|---|---|---|---|
| description For use in molecular biology applications | description United States Pharmacopeia (USP) Reference Standard | description analytical standard | description - |
| assay ≥98% (HPLC) | assay - | assay ≥95.0% (HPLC) | assay ≥99% (HPLC) |
| form powder | form - | form - | form crystalline solid |
| Quality Level 200 | Quality Level - | Quality Level 100 | Quality Level 100 |
| storage temp. room temp | storage temp. 15-25°C | storage temp. - | storage temp. 10-30°C |
| solubility ethanol: soluble | solubility - | solubility - | solubility DMSO: 5 mg/mL |
| color white to off-white | color - | color - | color white to off-white |
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Parola di avvertenza
Warning
Codici di pericolo
Avvertenze di sicurezza
Hazard Classifications
Acute Tox. 4 Dermal
Classe di stoccaggio
11 - Combustible Solids
Che succede?
WGK 3
DPI (dispositivi di protezione individuale)
dust mask type N95 (US), Eyeshields, Gloves
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How can i prepare 500uM Forskolin (10mg) and where can the prepared solution be stored?
1 risposta-
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What is the shelf life of the solid form of forskolin?
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This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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Will residual solvents inhibit forskolin activation of adenylate cyclase?
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Various solvents, including ethanol, inhibit the forskolin activation of adenylate cyclase. DMSO is recommended for preparing forskolin solutions, because at concentrations of 5% or less of DMSO, there is little if any inhibition of forskolin activation.
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How should I store a DMSO or ethanol solution of Product F3917, Forskolin?
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Forskolin solutions at 5 mg/mL in either ethanol or DMSO have been found to be stable for at least 6 months at room temperature, as determined by HPLC analysis.
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How can I make an aqueous solution of Product F3917, Forskolin?
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Forskolin is soluble in water (with 2% ethanol) up to 0.2 mM by first dissolving in ethanol at 5 mg/mL and performing subsequent dilutions in water. Similar results were obtained with DMSO.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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