F0556
Human Fibronectin
from human fibroblasts, liquid, ~0.5 mg/mL, suitable for cell culture
Sinonimo/i:
Fibronectin
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100 μL
333,00 €
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Cambia visualizzazione
100 μL
333,00 €
About This Item
Nome del prodotto
Fibronectina, cell culture derived, ~0.5 mg/mL, sterile-filtered, BioReagent, suitable for cell culture
Origine biologica
human fibroblasts
Livello qualitativo
Sterilità
sterile-filtered
Nome Commerciale
BioReagent
Stato
solution
PM
31 kDa
Confezionamento
pkg of 100 μL
Concentrazione
~0.5 mg/mL
tecniche
cell culture | mammalian: suitable
Impurezze
HBSAG, none detected
HCV, none detected
HIV-1/HIV-2, none detected
N° accesso NCBI
N° accesso UniProt
Specificità del legame
Peptide Source: Collagen
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
Informazioni sul gene
human ... FN1(2335)
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Descrizione generale
Fibronectin 1 is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35.[1]
Applicazioni
Fibronectin solution from human fibroblasts is used for the following applications:
Fibronectin from human plasma can be used with epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, and endothelial cells. It is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.
- It is used in purification of human extracellular matrix (ECM)[4]
- Coating of tissue culture plates with extracellular matrix components[5]
- Used during cell culture (For xeno-free, feeder-free culture the cells were passaged in to human fibronectin along with other components)[6]
Fibronectin from human plasma can be used with epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, and endothelial cells. It is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.
Azioni biochim/fisiol
Fibronectin is a high molecular mass extracellular matrix glycoprotein involved in many cellular processes, including tissue repair, embryogenesis, blood clotting, cell migration/adhesion, and embryogenesis and development. It is used in vitro as a substrate to enhance adherence and proliferation of many cell types. It can bind to macromolecules such as collagen, fibrin, glycosaminoglycans, and Clq, apart from some bacteria.[2]It serves as a critical factor in promoting cell survival and preventing programmed cell death in these cellular contexts.[7] Fibronectin plays a significant role in the wound healing process, and in extracellular matrix (ECM) assembly hence is suitable for tissue engineering applications.[8]
Componenti
Fibronectin is an adhesive glycoproteins found in both the cell surface and in plasma.
Avvertenza
The solution should be stored at -20°C. Under these conditions, the product is stable for at least 2 years. Vortexing, excessive agitation, or additoinal freezing and thawing of reconstituted fibronectin are not recommended.
Nota sulla preparazione
This product is produced by human fibroblasts and then purified biochemically. It is supplied as a sterile solution at 0.5 mg/mL in a CAPS saline buffer. Before use, the product should be thawed slowly at 2-8°C, forming a clear solution. Avoid any vortexing or vigorous shaking of the solution, which will cause any fibronectin to “crash” out of solution. To coat plates, dilute this product in sterile HBSS solutions and coat with a minimal volume. Then, incubate at 37°C for 1-2 hours and wash 3 times with sterile HBSS and plate cells.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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Nathalie Chênais et al.
Scientific reports, 9(1), 2861-2861 (2019-03-01)
Reprogramming of cultured cells using Xenopus egg extract involves controlling four major steps: plasma membrane permeabilization, egg factors import into the nucleus, membrane resealing, and cell proliferation. Using propidium iodide to assess plasma membrane permeability, we established that 90% of
J R Potts et al.
Current opinion in cell biology, 6(5), 648-655 (1994-10-01)
Significant progress has been made recently in the determination of the structure and assembly of the important matrix protein fibronectin, a molecule mainly constructed from three modular units denoted Fn1, Fn2 and Fn3. Atomic resolution structures are now available for
Sajjad Ahmad et al.
Stem cells (Dayton, Ohio), 25(5), 1145-1155 (2007-01-27)
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A Garcia-Pardo et al.
The Journal of biological chemistry, 260(18), 10320-10325 (1985-08-25)
The 31-kDa domain of human plasma fibronectin has been completely characterized. This fragment is located at the COOH-terminal end of the molecule immediately preceding the 3-kDa interchain disulfide-containing peptide. The 31-kDa domain was obtained after trypsin digestion of fibronectin and
Articoli
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
Protocolli
Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.
Filtri attivi
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