EPI001
Histone Acetyltransferase (HAT) Activity Assay Kit
100 assays in 96 well plates
Sinonimo/i:
HAT Activity, Histone Acetyltransferase Activity
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Prodotti consigliati
impiego
100 assays in 96 well plates
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
Descrizione generale
Histone acetyltransferases (HATs) have critical roles in cellular functions and are involved in the regulation of gene transcription, differentiation, and proliferation. The HAT Activity Colorimetric Assay Kit offers a convenient, non-radioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit includes an active Nuclear Extract (NE) to be used as a positive control, HAT cofactor, acetyl-CoA, and all downstream cofactors and substrates. Acetylation of a peptide substrate by active HAT releases the free form of CoA, which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous, and therefore, is suitable for kinetic studies. The kit provides all reagents and a simple, straightforward protocol for a complete assay.
Caratteristiche e vantaggi
- Sensitive and reliable assay
- Utilizes colorimetric methods
- Sample type: cell and tissue lysates
- Species reactivity: mammalian
- Suitable for individual tests or high throughput assays and kinetic studies
- Easy and simple procedure: combine nuclear extract or protein sample with reagents, incubate, and read absorbance
- Convenient 96-well microplate format
- Continuously measures HAT activity; suitable for kinetic studies
- No HDAC interference; crude nuclear extract can be used directly
Prodotti correlati
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Sydney P Thomas et al.
eLife, 10 (2021-10-23)
Short-chain fatty acids (SCFAs) acetate, propionate, and butyrate are produced in large quantities by the gut microbiome and contribute to a wide array of physiological processes. While the underlying mechanisms are largely unknown, many effects of SCFAs have been traced
Hiroshi Watanabe et al.
Nihon rinsho. Japanese journal of clinical medicine, 71(12), 2091-2096 (2014-01-21)
Aging alters a broad spectrum of physiological, endocrine, and behavioral rhythms, and the close relationship between age-associated disease and disrupted circadian rhythms has been shown. Circadian rhythms are regulated by a set of clock genes and mutations in clock genes
Michiko Konno
Nihon rinsho. Japanese journal of clinical medicine, 71(12), 2171-2178 (2014-01-21)
Mood disorders show a common feature of distorted cycling of biological systems, manifesting, for example, as diurnal mood variation in depression, phasic time course in bipolar disorder, and seasonal mood swing in seasonal affective disorder. Accordingly, circadian dysfunction has been
Koji Maemura
Nihon rinsho. Japanese journal of clinical medicine, 71(12), 2124-2129 (2014-01-21)
Cardiovascular function exhibits circadian oscillation. Furthermore, the onset time and severity of cardiovascular diseases exhibit diurnal variation. The internal circadian clock whose center exists in the suprachiasmatic nucleus regulates this circadian variation. Many studies have reported that night shift workers
Carolina Alquezar et al.
Science advances, 7(45), eabg3897-eabg3897 (2021-11-06)
Age-associated neurodegenerative disorders demonstrating tau-laden intracellular inclusions are known as tauopathies. We previously linked a loss-of-function mutation in the TSC1 gene to tau accumulation and frontotemporal lobar degeneration. Now, we have identified genetic variants in TSC1 that decrease TSC1/hamartin levels
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.