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EC600

Sigma-Aldrich

GenElute-E Single Spin DNA Cleanup Kit

greener alternative

Sinonimo/i:

E Single Spin DNA Cleanup Kit, GenElute DNA Cleanup Kit

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About This Item

Codice UNSPSC:
41105600
NACRES:
NA.52

Purificato mediante

(Single-spin negative chromotography)
(Time: 3 minutes or less)

Caratteristiche

Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS)
Intended use (For depletion of impurities and partial fractions (<50 bp) from DNA solutions)
Typical/expected yield (Varies by sample. Please reference user guide for more information.)

Caratteristiche più verdi

Waste Prevention
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

tecniche

DNA purification: suitable

Categoria alternativa più verde

Temperatura di conservazione

room temp

Descrizione generale

The GenElute-E Single Spin DNA Cleanup Kit is a one-step cleanup kit for the depletion of impurities and partial fractions (<50 bp) from pre-purified samples of DNA. The kit efficiently removes salt, detergent, nucleotides, primers, proteins/enzymes, organic solvents and many other inhibitors of downstream applications. The standard protocol, which can be completed in 3 minutes or less, is suitable for DNA fragments, plasmid DNA, and genomic DNA.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is aligned with "Waste prevention" and "Designing safer chemicals". Click here for more information.

Applicazioni

Traditional methods for purifying genomic DNA rely on medium and large format silica (bind-wash-elute) purification kits, which suffer from limited binding capacity, low DNA concentration, and low purity. The GenElute-E Single Spin DNA Clean-up Kit, depletes impurities such as salts, detergents, nucleotides, primers, proteins/enzymes, and dyes from DNA solutions. Purified genomic DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including PCR, genotyping, NGS, and others. Genomic DNA preparations usually show an A260/280 ratio of 1.8 to 2.0.

Caratteristiche e vantaggi

Fast and efficient purification (less than 3 minutes): single-spin protocol reduces pipetting steps and hands-on timeHigher quality DNA: negative chromotography-based purification reduces carryover of PCR inhibitors and other contaminents Reduced environmental impact: 55% less plastic waste, as well as a significant reduction in hazardous chemicals

Stoccaggio e stabilità

Use within 12 months of receipt.

Altre note

We believe that green chemistry will contribute to a better tomorrow. With a growing portfolio of greener alternatives, there are now more choices to reduce the ecological impact of your research while still delivering quality and efficacy so your results are not compromised.  GenElute-E purification kits avoid the use of hazardous wash buffers typically employed in silica-based purification kits, significantly reducing chemical waste. The single-spin protocol also limits the number of collection steps, reducing plastic waste by 55% compared to equivalent bind-wash-elute methods. GenElute-E is designated a Greener Alternative Product based on the "Prevention" and "Designing Safer Chemicals" qualifications of The 12 Principles of Green Chemistry.GenElute-E kits also adhere to the principles of SMASH Packaging, our global initiative to improve the sustainability through the reduction of packaging materials, the increased use of sustainable alternatives, and improved recyclability. Packaging is FCS-marked for sustainable forestry practices and produced from greater than 70% recycled content.

Note legali

GenElute is a trademark of Sigma-Aldrich Co. LLC

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3


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Articoli

GenElute™-E DNA purification kits ensure accurate DNA quantitation and downstream enzymatic processes.

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