D9281

Lambda DNA EcoR I Hind III Digest

for DNA electrophoresis

• Codice UNSPSC: 41141827
• NACRES: NA.25

Proprietà

• Stato: liquid
• impiego: 100 uses (0.1 mg size); 500 uses (0.5 mg size)
• Compatibilità: suitable for electrophoresis (DNA)
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Sigma′s Lambda DNA EcoR I Hind III Digest contains 13 fragments from 125 – 21,226 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Applicazioni

Lambda DNA EcoR I Hind III Digest has been used as DNA markers for comparing the molecular weights of polymerase chain reaction (PCR) amplified products.[1]

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Componenti

Sigma′s Lambda DNA EcoR I Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Altre note

Contains 13 fragments, 125–21,226 bp

For optimal resolution, the recommended agarose gel concentration is 0.7%.

Nota sulla preparazione

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H317
• Consigli di prudenza: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classi di pericolo: Skin Sens. 1
• Codice della classe di stoccaggio: 13 - Non Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable