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D8312

Sigma-Aldrich

REDTaq® Genomic DNA Polymerase

with MgCl2

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About This Item

Numero MDL:
MFCD01635818
Codice UNSPSC:
12352204
NACRES:
NA.55

Forma fisica

liquid

impiego

sufficient for 1000 reactions
 sufficient for 250 reactions
 sufficient for 2500 reactions

Caratteristiche

Difficult Templates/Specialty Enzymes PCR
dNTPs included: no
hotstart: no

Concentrazione

1 unit/μL

tecniche

PCR: suitable

Colore

red

input

purified DNA

applicazioni

agriculture

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

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Descrizione generale

REDTaq® Genomic DNA Polymerase is a unique blend of Taq DNA Polymerase with an inert red dye. This special formulation is designed to provide enhanced amplification of more complex or genomic templates. REDTaq Genomic DNA Polymerase is highly sensitive, produces increased yields and is capable of generating longer product lengths. It has all the advantages of REDTaq DNA polymerase, such as easy visualization of enzyme addition and complete reaction mixing, and direct loading to an agarose gel. The inert red dye does not effect automated or manual sequencing, restriction digestions or other downstream applications. The dye can easily removed by any standard purification method.
The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides (the building blocks of DNA). These enzymes are essential for DNA replication and usually work in pairs. It creates two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.

Applicazioni

REDTaq® Genomic DNA Polymerase is used for routine PCR amplification of genomic DNA.

Caratteristiche e vantaggi

  • Enhanced amplification on genomic and difficult DNA templates
  • Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications
  • Quick recognition and confirmation of appropriate mixing
  • No loading buffers or tracking dyes necessary. Sample can be taken directly from reaction and loaded onto an agarose gel
  • The samples can be re-amplified as in nested PCR
  • The red dye migrates faster than bromophenol blue
  • Greater consistency across reactions due to proper mixing

Definizione di unità

One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min. at 74°C.

Note legali

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS
  • P219210X PCR BufferSDS

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Determinazione del prezzo

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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J A F Demandt et al.
Scientific reports, 11(1), 425-425 (2021-01-13)
Hypoxia is prevalent in atherosclerotic plaques, promoting plaque aggravation and subsequent cardiovascular disease (CVD). Transmembrane protein carbonic anhydrase IX (CAIX) is hypoxia-induced and can be shed into the circulation as soluble CAIX (sCAIX). As plaque macrophages are hypoxic, we hypothesized a
Preparation of genomic DNA from Dictyostelium discoideum for PCR analysis.
Steve J Charette et al.
BioTechniques, 36(4), 574-575 (2004-04-20)
L M Winton et al.
Phytopathology, 92(1), 112-116 (2008-10-24)
ABSTRACT Phaeocryptopus gaeumannii is a widespread foliar parasite of Douglas-fir. Although normally innocuous, the fungus also causes the defoliating disease Swiss needle cast in heavily infected needles. The extent of P. gaeumannii colonization in Douglas-fir foliage was estimated with real-time
Genotyping of HLA-B27 by real-time PCR without hybridization probes.
M A Bon et al.
Clinical chemistry, 46(7), 1000-1002 (2000-07-15)
Gregory G Martin et al.
The Journal of biological chemistry, 278(24), 21429-21438 (2003-04-03)
Although liver fatty acid-binding protein (L-FABP) is an important binding site for various hydrophobic ligands in hepatocytes, its in vivo significance is not understood. We have therefore created L-FABP null mice and report here their initial analysis, focusing on the
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Protocolli

Applications with REDTaq™

Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.

Amplification of Genomic DNA using REDTaq® DNA Polymerase

Reactions using REDTaq® DNA polymerase are formulated as any PCR mixtures. There are no additional reaction preparation steps or protocol changes required.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.