A clone derived from the parental HSDM1 cell line established from a transplantable fibrosarcoma of a Swiss Albino mouse. The cells secrete prostaglandin E2 which is inhibited by aspirin like drugs such as indomethacin.
Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
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