L-M (TK-)

90083001, mouse connective tissue, Fibroblast

• Codice UNSPSC: 41106514

Proprietà

• product name: L-M (TK-), 90083001
• Origine biologica: mouse connective tissue
• Modalità di accrescimento: Adherent
• Cariotipo: Modal no. 45; hyperdiploid
• Morfologia: Fibroblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse C34/An connective tissue

Descrizione della linea cellulare

Derived from a sub-line of a BUdR resistant strain of the L-M mouse fibroblast cell line. This cell line has potential for use in genetic and biochemical studies including somatic cell hybridisation and gene transfer.

Applicazioni

Gene transfer, genetic, biochemical and hybridisation studies

Terreno di coltura

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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