P22

89030317, hamster ovary, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• Nome del prodotto: P22, 89030317, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
• Origine biologica: hamster ovary
• Confezionamento: tube of 5 μg 89030317-DNA-5UG; pkg of vial of cells 89030317-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Hamster Chinese ovary

Descrizione della linea cellulare

Chinese hamster ovary cell line which lacks ornithine decarboxylase activity.

Terreno di coltura

EMEM (EBSS) + Ham′s F12 (1:1) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 0.1% BSA.

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.1% cold PBS and 0.25% cold trypsin. Place flasks at 4°C for 5-10 min. or until cells detach; 5% CO₂; 37°C. Pre-treat culture flasks with porcine gela

Altre note

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