W3/25

Reactivity: Anti-CD4, rat, 84112002

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• Mieloma: NS1/1
• Reattività: Anti-CD4, rat
• tecniche: cell culture | mammalian: suitable
• Isotipo: IgG1
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse NS1/1 x Mouse BALB/c spleen

Descrizione della linea cellulare

The antibody is directed against surface antigens found on 86% of rat thymocytes, 70% of peripheral T lymphocytes and macrophages. The antigen has a molecular weight of approximately 50kDa. W3/25 positive T cells have been identified as T helper cells.

Immunogeno

Rat thymocyte membrane

Terreno di coltura

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Mantenimento delle subcolture

Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO₂; 37°C.

Altre note

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Esclusione di responsabilità

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.