BHK21C13-3P

84100501, hamster kidney, Fibroblast

• Codice UNSPSC: 41106514

Proprietà

• product name: BHK21C13-3P, 84100501
• Origine biologica: hamster kidney
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 44, pseudodiploid
• Morfologia: Fibroblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Hamster Syrian kidney

Descrizione della linea cellulare

BHK21C13-3P is a subclone of the parent line BHK-21 (Clone-13) (Sigma Catalogue number. 85011433). The cells grow as monolayers and have the same virus susceptibility as the parent line.

Applicazioni

Virus replication studies

Terreno di coltura

GMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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