Ma-Mel-45a

13012486, human skin, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• Nome del prodotto: Ma-Mel-45a, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., from human skin, 13012486
• Origine biologica: human skin
• Descrizione: Human melanoma, metastasis
• Confezionamento: tube of 5 μg 13012486-DNA-5UG; pkg of vial of cells 13012486-1VL
• Modalità di accrescimento: Adherent
• Morfologia: Epithelial
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Descrizione della linea cellulare

Ma-Mel-45a (ESTDAB-173) was established from the brain metastasis in a 39 year-old male patient with metastatic melanoma (unknown primary stage IV). The Ma-Mel-45a cell line is one of more than 170 characterized melanoma cell lines collected during the ESTDAB project (European Searchable Tumour Line Database) part of the European Commissions fifth framework infrastructures programme (contract no. QLRI-CT-2001-01325). ESTDAB contains information on HLA genotype and surface expression of HLA molecules, expression of tumour antigens, antigen processing capability, production of and response to cytokines and chemokines, apoptosis regulation and expression of adhesion/accessory molecules. Such information allows researchers to identify cells possessing specific parameters important for studies of immunity, immunogenetics, gene expression, metastasis, response to chemotherapy, and other tumour biological experimentation.

Profilo DNA

STR-PCR Data:

Amelogenin: X
CSF1PO: 10,11
D13S317: 8
D16S539: 12
D5S818: 12
D7S820: 9,13
THO1: 7,8
TPOX: 11
vWA: 16,18

Terreno di coltura

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) seed new cultures at 1-3 x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density approx 5 x10,000 cells/cm² .

Altre note

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.