Calculating transfection efficiency is very useful when optimizing transfection protocols. Transfection efficiency can be performed using a GFP-expressing plasmid. After transfection, cells are stained with propidium iodide and counted. The propidium iodide provides a count of the total cells in the population, and the GFP-expressing cells provide a count of the number of cells transfected. The transfection efficiency (%) can then be calculated by:(# GFP-expressing cells / total cell #) * 100
CAPHOS
Calcium Phosphate Transfection Kit
Most cost effective transfection reagent kit for transient and stable transfection of DNA into mammalian cells
Sinonimo/i:
Gene delivery
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| A voi/SKU | Disponibilità | Prezzo |
|---|---|---|
1 kit | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 627,00 € |
Informazioni su questo articolo
UNSPSC Code:
12352200
EC Number:
233-140-8
NACRES:
NA.85
Form:
solution
Grade:
Molecular Biology
Technique(s):
transfection: suitable
627,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Servizio Tecnico
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Permettici di aiutartigrade
Molecular Biology
Quality Segment
form
solution
usage
kit sufficient for 160 transfections (6 cm dishes), kit sufficient for 400 transfections (3.5 cm dishes), kit sufficient for 80 transfections (10 cm dishes)
technique(s)
transfection: suitable
shipped in
dry ice
storage temp.
−20°C
General description
Calcium phosphate transfection is a commonly used method for the introduction of DNA into eukaryotic cells. This technique has been used to obtain both transient and stable transfections in a wide variety of cell types.
Application
Calcium Phosphate Transfection Kit has been used:
- to enable transfection
- to transfect Hek293T cells
- to transfect H29D cells
Suitable for transient and stable transfection of DNA into cultured mammalian cells. The following cells have successfully been transfected using the calcium phosphate method:
BAEC
Bowels melanoma cells
CHO K1
COS-7
Fibroblasts (human embryonic, neo derm)
HEK293
Huh 7
IMR-90
LLC (Lewis Lung Carcinoma)
NIH3T3
PC-12
PCI-13
SH-Sy5Y
SK-Hep-1
T47D
BAEC
Bowels melanoma cells
CHO K1
COS-7
Fibroblasts (human embryonic, neo derm)
HEK293
Huh 7
IMR-90
LLC (Lewis Lung Carcinoma)
NIH3T3
PC-12
PCI-13
SH-Sy5Y
SK-Hep-1
T47D
Biochem/physiol Actions
The procedure is based on slow mixing of HEPES-buffered saline containing sodium phosphate with a CaCl2 solution containing the DNA. A DNA-calcium phosphate co-precipitate forms, which adheres to the cell surface and is taken up by the cell, presumably by endocytosis.
Features and Benefits
- Suitable for transient and stable transfection
- Reproducible for a wide range of cell types
- Widely referenced
- Inexpensive
Other Notes
The Calcium Phosphate Transfection Kit contains:
5 ml 2.5M CaCl2 (C2052)
25 ml 2x HEPES Buffered Saline (H1012)
25 ml molecular biology grade water (W4502)
5 ml 2.5M CaCl2 (C2052)
25 ml 2x HEPES Buffered Saline (H1012)
25 ml molecular biology grade water (W4502)
The protocol was developed for transient transfection of CHO cells using pSV40-CAT plasmid as a reporter gene.
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Questo articolo | |||
|---|---|---|---|
| description Most cost effective transfection reagent kit for transient and stable transfection of DNA into mammalian cells | description Lipid formulation for nucleic acid transfections in neuronal and glial cells | description - | description Avanti Research™ - A Croda Brand |
| technique(s) transfection: suitable | technique(s) transfection: suitable | technique(s) microbe id | staining: suitable | technique(s) transfection: suitable |
| form solution | form dried film | form - | form powder |
| grade Molecular Biology | grade Molecular Biology | grade - | grade - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in - | shipped in dry ice | shipped in dry ice |
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Classe di stoccaggio
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 1
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How can I determine the efficiency of my transfection?
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Is optimizing the transfection protocol important?
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For many common cell lines, transfection reagent efficiency is very high and the protocols will not require any optimization. For hard-to-transfect cells or those ultimately expressing a toxic protein, the protocol should be optimized for best transfection efficiency. Taking time to optimize will give you more transfected cells with each procedure, which can mean more protein expressed and results that are reproducible.
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What is transfection efficiency?
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Transfection efficiency is a measure of how many cells take up the DNA during the transfection process. Many transfection reagents can achieve a transfection efficiency of >90% in common cell lines. Other cell lines are hard to transfect, and require special reagents and/or techniques to achieve even a small population of transfected cells.
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What is the difference between stable and transient transfection?
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When the DNA enters the nucleus of the cell, the plasmid is replicated by the cell machinery (transient transfection). During this time, RNA is transcribed and protein translated until the plasmid DNA is lost after a few cell divisions. This expression of the plasmid DNA, mRNA, and protein is transient (temporary).In some cases, the plasmid DNA is integrated into the host cell genome. This is usually accompanied by forced expression using a selection antibiotic and sometimes a cloning step (to be sure all cells have the same integration site). Once the DNA is stable, the cell line can be frozen and used to express protein for many years. Clones may even be screened for those expressing the highest amount of protein.
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How can I increase the efficiency of my transfection?
1 risposta-
Transfection efficiency is affected by many different things, including plasmid size and purity, media components present, transfection reagent selected, amount of DNA and transfection reagent used, cell density, etc. Optimizing the protocol with respect to these concerns will allow you to achieve a higher transfection efficiency. For many cell lines and transfection reagents, optimized protocols are already available.
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What is the Department of Transportation shipping information for this product?
1 risposta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is the size of the plasmid an important consideration for transfection?
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The size of the plasmid should be considered when selecting a transfection reagent with the best efficiency. In general, larger sized plasmids should easily transfect with readily available transfection reagents, as along as the plasmid DNA is of high purity.
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Can I transfect cells plated at low density?
1 risposta-
For most transfections, cells should be >70% confluency the day of transfection, and growing in mid-log phase. Some transfection reagents are now designed to work with cells at low density, when required.
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Can antibiotics be present in the medium during transfection?
1 risposta-
We recommend that no antibiotics are present during transfection. The process of transfection can make the cells somewhat more porous to allow for efficient DNA entry. During this time, antibiotics will also enter the cells more easily and the cells may show increased cell death. Wait until about 24 hours after transfection to resume the use of preventative antibiotics and/or start the use of selective antibiotics.
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Why do I see a precipitate in my cell culture after calcium-phosphate transfection?
1 risposta-
The precipitate is normal to see - this is the calcium/DNA precipitate the cell will internalize during the transfection process. If bubbled correctly, the precipitates will be very fine and regular sized, then evenly distributed over the cells.
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