C-28053

Dendritic Cell Generation Medium

Basal Medium, 250 ml

• Codice UNSPSC: 12352207
• NACRES: NA.71

Proprietà

• Stato: liquid
• Confezionamento: pkg of 250 ml
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

DC Base Medium (supplements sold separately)

Applicazioni

The Dendritic Cell (DC) Base Medium is a user-customizable version of the DC Generation Media that does not include cytokines. It must be adequately supplemented by the user for the generation of immature as well as fully mature myeloid Dendritic Cells from peripheral blood monocytes.Note: DC Base Medium must be supplemented with additional cytokines. For freshly isolated cells, the Monocyte Attachment Medium (C-28051) is also needed.Recommended for:Human Monocytes (hMo), freshly isolated and cryopreservedHuman Mononuclear Cells (hMNC), freshly isolated

Qualità

All lots of PromoCell DC Media are subjected to comprehensive quality control tests using human peripheral blood mononuclear cells. Each lot of PromoCell DC Media is tested for the ability to support DC maturation verified by morphological evaluation and flow cytometry analysis. Each lot of PromoCell Monocyte Attachment Medium is tested for its ability to support optimal attachment and viability of peripheral blood Monocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Stoccaggio e stabilità

Store the Basal Medium at 4 to 8°C in the dark immediately after arrival. Do not freeze the Basal Medium. If stored properly, the medium is stable until the expiry date stated on the label. After adding the SupplementMix to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Complete Medium supplemented with cytokines should be used within 2 weeks. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Mantenimento delle subcolture

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Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable