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B7786

Sigma-Aldrich

Anti-fd Bacteriophage antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Sinonimo/i:

Anti-fd Bacteriophage

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.46

Origine biologica

rabbit

Coniugato

unconjugated

Forma dell’anticorpo

IgG fraction of antiserum

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Forma fisica

buffered aqueous solution

Confezionamento

antibody small pack of 25 μL

tecniche

indirect ELISA: 1:1,000 using 5 × 1010 phage/ml

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

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Specificità

The antibody binds specifically to phage coat proteins of fd phage or M13 phage and thus may act as a capture antibody when coated directly on multiwell plates or as a primary detection antibody for specifically captured fd or M13 phage. The antibody may be useful in rapidly sorting large phage display libraries (antibody, peptide, etc.) with the expressed proteins fused to either the gene III or to the gene VIII protein of the filamentous phage. The antibody may be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.

Immunogeno

fd bacteriophage

Applicazioni

Anti-fd Bacteriophage antibody produced in rabbit may be used in ELISA at a dilution of 1:1000. It was used to in antiphage immunohistochemistry.
B7786 Rabbit Anti-fd Bactieriophage may be used to sort phage display libraries (antibody, peptide, etc.) with expressed proteins fused to either the gene III or gene VIII protein of filamentous bacteriophage. The product may also be used in "phage ELISA" offering specific activity for detection of recombinant bacteriophage. It will also function as a capture antibody when coated directly on microwell plates or as primary detection antibody for specifically captured fd or M13 bacteriophage.

Stato fisico

Solution supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Nota sulla preparazione

Anti-fd Bacteriophage is developed in rabbit using repeated injections of fd bacteriophage as the immunogen. Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum which is essentially free of other rabbit serum proteins.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 20(7), 979-981 (2006-04-04)
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