B3795

Brain-derived neurotrophic factor human

≥95% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Sinonimo/i: Abrineurin, BDNF
• Numero MDL: MFCD00285579
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Nome del prodotto: Brain-derived neurotrophic factor human, BDNF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• Origine biologica: human
• Livello qualitativo: 200
• Ricombinante: expressed in E. coli
• Saggio: ≥95% (SDS-PAGE)
• Stato: lyophilized powder
• Qualità: endotoxin tested
• PM: ~27 kDa
• Confezionamento: pkg of 10 μg; pkg of 5 μg
• Condizioni di stoccaggio: avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
• tecniche: cell culture | mammalian: suitable
• Impurezze: ≤1.00 EU/μg Endotoxin level
• N° accesso UniProt: P23560
• Temperatura di conservazione: −20°C
• Informazioni sul gene: human ... BDNF(627) , BDNF(627)

Descrizione

Descrizione generale

BDNF is a member of the neurotrophin family of growth factors that includes NGF, NT-3, and NT-4. All neurotrophins have six conserved cysteine residues and share a 55% sequence identity at the amino acid level. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.1 BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non-neuronal cells.
The active form of BDNF recombinant human protein (27 kDa) is a dimer formed by two identical 119 amino acid subunits held together by strong hydrophobic interactions

Applicazioni

Brain-derived neurotrophic factor (BDNF) recombinant human protein has been used as a supplement in various types of culture media.[1][2][3]

Azioni biochim/fisiol

Brain-derived neurotrophic factor (BDNF) is a 13.6 kDa (or 27.2 kDa dimer) member of the neurotrophin family. BDNF has identical amino acid sequence in human, mouse, and pig with full cross-reactivities. BDNF is important in development and maintenance of neuronal populations within the central nervous system or cells directly associated with it. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.

Stato fisico

lyophilized from a sterile 0.2 μm filtered solution with BSA

Risultati analitici

The biological activity is determined by its ability to stimulate the proliferation of human neuroblastoma cells, SH-SY5Y.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)