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B1064

Sigma-Aldrich

Cibacron Blue 3G-A

Dye content ≥55 %, Powder

Sinonimo/i:

Cibacron Blue; 1-amino-4-[4-[[4-chloro-6-(2-sulfoanilino)-1,3,5-triazin-2-yl]amino]-3-sulfoanilino]-9,10-dioxoanthracene-2-sulfonic acid

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About This Item

Formula empirica (notazione di Hill):
C29H20ClN7O11S3
Numero CAS:
84166-13-2
Peso molecolare:
774.16
Codice UNSPSC:
12171500
NACRES:
NA.47

product name

Cibacron Blue 3G-A, Dye content ≥55 %

Forma fisica

powder

Composizione

Dye content, ≥55%

Colore

dark blue

Solubilità

H2O: 10 mg/mL, blue

applicazioni

diagnostic assay manufacturing
hematology
histology

Temperatura di conservazione

room temp

Stringa SMILE

Nc1c(cc(Nc2ccc(Nc3nc(Cl)nc(Nc4ccccc4S(O)(=O)=O)n3)c(c2)S(O)(=O)=O)c5C(=O)c6ccccc6C(=O)c15)S(O)(=O)=O

InChI

1S/C29H20ClN7O11S3/c30-27-35-28(33-16-7-3-4-8-19(16)49(40,41)42)37-29(36-27)34-17-10-9-13(11-20(17)50(43,44)45)32-18-12-21(51(46,47)48)24(31)23-22(18)25(38)14-5-1-2-6-15(14)26(23)39/h1-12,32H,31H2,(H,40,41,42)(H,43,44,45)(H,46,47,48)(H2,33,34,35,36,37)
YKCWQPZFAFZLBI-UHFFFAOYSA-N

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Categorie correlate

Applicazioni

When immobilized using an insoluble porous support matrix such as agarose, Cibacron Blue 3GA is used for affinity chromatography purification of enzymes. This affinity has been attributed to a structural similarity between the dye and the natural ligands for proteins with cofactor binding domains.

Azioni biochim/fisiol

Cibacron Blue 3GA is an anionic anthraquinone dye. It acts as a P2-purinoceptor antagonist and inhibits stimulus-evoked glutamate release by rat brain cortical tissue. Cibacron Blue 3GA inhibits OXA-1 and OXA-2 β-lactamases and was used to observe the binding of ligands to OXA-1 β-lactamase by monitoring tryptophan fluorescence.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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W O Smith et al.
Plant physiology, 68(2), 443-446 (1981-08-01)
The binding of phytochrome to Cibacron Blue 3GA was utilized to develop a new affinity purification procedure for phytochrome. Brushite-purified phytochrome from rye (Secale cereale c.v. Cougar) was bound to agarose-immobilized blue dye in 0.1 molar potassium phosphate (pH 7.8)
I von Kügelgen et al.
British journal of pharmacology, 113(3), 815-822 (1994-11-01)
1. Some postganglionic sympathetic axons possess P2Y-like P2-purinoceptors which, when activated, decrease the release of noradrenaline. We examined the question of whether such receptors also occur at the noradrenergic axons in the rat brain cortex. Slices of the brain cortex
S T Thompson et al.
Proceedings of the National Academy of Sciences of the United States of America, 72(2), 669-672 (1975-02-01)
A procedure is described to utilize blue dextran-Sepharose as an affinity chromatographic column specific for the super-secondary structure called the dinucleotide fold, which forms the binding sites for substrates and effectors on a wide range of proteins. The procedure can
G C Bennett et al.
British journal of pharmacology, 131(3), 617-623 (2000-10-04)
Evidence has previously been presented that P1 receptors for adenosine, and P2 receptors for nucleotides such as ATP, regulate stimulus-evoked release of biogenic amines from nerve terminals in the brain. Here we investigated whether adenosine and nucleotides exert presynaptic control
Kyle D Schneider et al.
Biochemistry, 48(26), 6136-6145 (2009-06-03)
Class D beta-lactamases hydrolyze beta-lactam antibiotics by using an active site serine nucleophile to form a covalent acyl-enzyme intermediate and subsequently employ water to deacylate the beta-lactam and release product. Class D beta-lactamases are carboxylated on the epsilon-amino group of
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