A5168

Adenosine Deaminase from bovine spleen

Type IX, ammonium sulfate suspension, 150-200 units/mg protein

• Sinonimo/i: Adenosine aminohydrolase
• Numero CAS: 9026-93-1
• Classificazione EC (Enzyme Commission): 3.5.4.4 (BRENDA, IUBMB)
• Numero CE: 232-817-5
• Numero MDL: MFCD00081287
• Codice UNSPSC: 12352204
• NACRES: NA.54

Proprietà

• Origine biologica: bovine spleen
• Livello qualitativo: 200
• Tipo: Type IX
• Forma fisica: ammonium sulfate suspension
• Attività specifica: 150-200 units/mg protein
• PM: 32.5-33 kDa
• N° accesso UniProt: P56658
• Attività estranea: 5′-AMP deaminase ≤0.01%; alkaline phosphatase ≤0.01%; guanase ≤0.01%; nucleoside phosphorylase ≤0.1%
• Temperatura di conservazione: 2-8°C
• Informazioni sul gene: cow ... ADA(280712)

Descrizione

Descrizione generale

Adenosine deaminase/ADA is an enzyme, that is mainly present in lymphocytes and monocytes.

Molecular weight: 32.5-33 kDa

pI: 4.85

Applicazioni

Adenosine Deaminase from bovine spleen has been used to metabolize adenosine triphosphate (ATP) and adenosine to an inactive form (inosine). It has also been used to as an additive in KRH (Krebs−Ringer solution buffered with Hepes, containing CaCl2) medium in order to remove exogenous adenosine for cyclic adenosine monophosphate (cAMP) assay.

Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.

Azioni biochim/fisiol

Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.

Adenosine deaminase/ADA can be used as an important marker to identify tuberculous pleural effusion. The activity of ADA is usually high in liver disease, tuberculosis, typhoid, infective mononucleosis and several malignancies.

Definizione di unità

One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.

Stato fisico

Suspension in 3.2 M (NH₄)₂SO₄, 0.01 M potassium phosphate, pH 6.0

Risultati analitici

Protein determined by biuret.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)