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A2765

Sigma-Aldrich

Azocasein

protease substrate, chromogenic, powder

Sinonimo/i:

Sulfanilamide-azocasein

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About This Item

Numero CAS:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.32

product name

Azocasein, protease substrate

Origine biologica

bovine

Forma fisica

powder

tecniche

ligand binding assay: suitable

Solubilità

water: 5 mg/mL, clear, orange to very deep orange

ε (coefficiente d’estinzione)

≥25 at 440 nm in 0.1 M NaOH at 1%

Temperatura di conservazione

2-8°C

Descrizione generale

Azocasein is a chromogenic derivative of casein. Protease degrades azocasein to yield TCA-soluble azopeptides with high UV-absorbance. This azocasein assay is widely employed to estimate the protease production by bacterial fermentation on synthetic substrates from glucose and inorganic salts.

Applicazioni

Azocasein has been used as a substrate for determination of protease activity.
Azocasein is a nonspecific protease substrate. Hydrolysis of the casein releases the azo dye into the media where it is detected by absorbance at 440 nm.
Azocasein is an inflammatory agent that is used to induce amyloid A amylooidosis in experimental animals.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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