A1935

Photobiotin acetate salt

For labeling DNA probes for hybridizations

• Sinonimo/i: Biotin {3-[3-(4-azido-2-nitroanilino)-N-methylpropylamino]propylamide} acetate salt, N-(4-Azido-2-nitrophenyl)-N′-(3-biotinylaminopropyl)-N′-methyl-1,3-propanediamine acetate salt
• Formula empirica (notazione di Hill): C₂₃H₃₅N₉O₄S · C₂H₄O₂
• Numero CAS: 96087-38-6
• Peso molecolare: 593.70
• Numero MDL: MFCD00210085
• Codice UNSPSC: 12352200
• ID PubChem: 24890604
• NACRES: NA.52

Proprietà

• Grado: for molecular biology
• Livello qualitativo: 200
• Saggio: ≥95%
• Forma fisica: film or powder
• Solubilità: H₂O: 10 mg/mL (Stable for at least 5 months if kept frozen and protected from light.)
• Temperatura di conservazione: −20°C
• Stringa SMILE: CC(O)=O.CN(CCCNC(=O)CCCC[C@@H]1SCC2NC(=O)NC12)CCCNc3ccc(cc3[N+]([O-])=O)N=[N+]=[N-]
• InChI: 1S/C23H35N9O4S.C2H4O2/c1-31(12-4-10-25-17-9-8-16(29-30-24)14-19(17)32(35)36)13-5-11-26-21(33)7-3-2-6-20-22-18(15-37-20)27-23(34)28-22;1-2(3)4/h8-9,14,18,20,22,25H,2-7,10-13,15H2,1H3,(H,26,33)(H2,27,28,34);1H3,(H,3,4)/t18-,20-,22-;/m0./s1; FFBLNTOMOSLSQM-AYEYRVMASA-N

Descrizione

Descrizione generale

Photobiotin acetate is made up of biotin, which is bound with the help of a charged linker arm to a photoreactive arylazide group.

Applicazioni

Photobiotin acetate salt has been used to label DNA probes to perform DNA−DNA hybridization to determine genomic relatedness.

Azioni biochim/fisiol

Photobiotin acetate is capable of labeling single stranded DNA and linear or supercoiled double-stranded DNA.

Photobiotin is a photo-activatable analog of biotin used primarily for labeling DNA, RNA and proteins. Probes prepared using photobiotin can detect target DNA as little as 0.5 pg.

Avvertenza

Light sensitive.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)