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78948

Sigma-Aldrich

Atto 425 azide

suitable for fluorescence

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About This Item

Formula empirica (notazione di Hill):
C30H43N5O8
Peso molecolare:
601.69
Codice UNSPSC:
12352200
ID PubChem:
329768198

Saggio

≥80.0% (HPCE)

Stato

solid

Produttore/marchio commerciale

ATTO-TEC GmbH

Fluorescenza

λex 436 nm; λem 484 nm±10 nm in PBS, pH 7.4

Compatibilità

suitable for fluorescence

Temperatura di conservazione

−20°C

Stringa SMILE

CC(CC(C)(C)N1CCCC(NCCOCCOCCOCCN=[N+]=[N-])=O)C(C1=C2)=CC3=C2OC(C(C(OCC)=O)=C3)=O

InChI

1S/C30H43N5O8/c1-5-42-28(37)24-18-22-17-23-21(2)20-30(3,4)35(25(23)19-26(22)43-29(24)38)10-6-7-27(36)32-8-11-39-13-15-41-16-14-40-12-9-33-34-31/h17-19,21H,5-16,20H2,1-4H3,(H,32,36)
RSOCGOAHHWBJFE-UHFFFAOYSA-N

Descrizione generale

Atto 425 is a fluorescent label with a coumarin structure. The dye is intended for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, high fluorescence quantum yield, large Stokes-shift, good photostability, and low molecular weight.
Atto 425 is moderately hydrophilic. Its fluorescence can be excited efficiently in the range 405 - 455 nm.
The azide modification is suitable for reactions with alkyne groups (Huisgen reaction - “Click Chemistry“).

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Note legali

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
BCBM6003V

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Katrin G Heinze et al.
Biophysical journal, 86(1 Pt 1), 506-516 (2003-12-26)
Confocal fluorescence spectroscopy is a versatile method for studying dynamics and interactions of biomolecules in their native environment with minimal interference with the observed system. Analyzing coincident fluctuations induced by single molecule movement in spectrally distinct detection channels, dual-color fluorescence
Triple FRET: a tool for studying long-range molecular interactions.
Elke Haustein et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 4(7), 745-748 (2003-08-07)
Anna I Sulatskaya et al.
The journal of physical chemistry. B, 116(8), 2538-2544 (2012-01-25)
Benzothiazole dye thioflavin T (ThT) is a sensitive probe for amyloid fibril detection. The ThT probing is based on its unique ability to form highly fluorescent complexes with amyloid and amyloid-like fibrils. In this work we propose an approach of
Anna I Sulatskaya et al.
The journal of physical chemistry. B, 115(39), 11519-11524 (2011-08-26)
The fluorescence of the benzothiazole dye thioflavin T (ThT) is a well-known test for amyloid fibril formation. It has now become evident that ThT can also be used for structural investigations of amyloid fibrils and even for the treatment of
Nermin Seda Kehr et al.
Nanoscale, 2(4), 601-605 (2010-07-21)
Microcontact printing (mCP) is used to immobilize dyes and peptides asymmetrically, by a "peptide coupling" reaction, on monolayers of zeolite L crystals in the contact area between the stamp and the surface of the monolayer. Chemically patterned surfaces of monolayers
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