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77730

Millipore

Gram Staining Kit

for microscopy, used for differentiation of bacteria on the basis of their gram nature.

Sinonimo/i:

Bacteria Staining Kit according to Gram

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About This Item

Codice UNSPSC:
41171621
NACRES:
NA.85

Grado

for microscopy

Livello qualitativo

100

agenzia

according to GB 4789.30-2016

Nome Commerciale

BioChemika

Durata

limited shelf life, expiry date on the label

tecniche

microbe id | staining: suitable

Spettro attività antibiotica

Gram-negative bacteria
Gram-positive bacteria

applicazioni

clinical testing
environmental
food and beverages
pharmaceutical

Compatibilità

bacteria

Descrizione generale

The Gram staining kit is used in differential staining technique that is most widely applied in all microbiology disciplines laboratories. It is one of the most important criteria in any identification scheme for all types of bacterial isolates. There are many physiological differences between gram-positive and gram-negative cell walls. Ever since Christian Gram has discovered Gram staining, this process has been extensively investigated and redefined. In practice, a thin smear of bacterial cells is stained with crystal violet, then treated with iodine containing mordant to increase the binding of the primary stain. A decolorizing solution of alcohol or acetone is used to remove the crystal violet from cells which bind it weakly and then the counterstain safranin is used to provide color contrast in those cells that are decolorized. In a properly stained smear by gram staining procedure, the gram-positive bacteria appear blue to purple and gram-negative cells appear pink to red

Applicazioni

The Gram staining method is one of the most important staining techniques in microbiology. It is also the most important criteria in any identification scheme for all types of bacterial isolates to differentiate the gram-positive and gram-negative bacteria.

Componenti

Kit contains (250ml each):
94448 Gram′s crystal violet Solution
90107 Gram′s iodine Solution
75482 Gram′s Decolorizer Solution
94635 Gram′s safranin Solution

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Avvertenze

Danger

Indicazioni di pericolo

H225,H319,H336,H351,H412

Classi di pericolo

Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

Organi bersaglio

Central nervous system

Rischi supp

EUH066

Codice della classe di stoccaggio

3 - Flammable liquids

Punto d’infiammabilità (°F)

68.0 °F

Punto d’infiammabilità (°C)

20 °C

Dispositivi di protezione individuale

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificati d'analisi (COA)

Lot/Batch Number
BCCM7421
BCCM5122
BCCM2797
BCCM0267
BCCL8176

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