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RMHMAG-84K

Millipore

MILLIPLEX® Rat Metabolic Hormone Magnetic Bead Panel - Metabolism Multiplex Assay

The analytes available for this multiplex kit are: Amylin (Active), C-Peptide 2, Ghrelin (Active), GIP (Total), GLP-1 (Active), Glucagon, IL-6, Insulin, Leptin, MCP-1, PP, PYY, TNF-α.

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About This Item

Codice UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.84

To order a Milliplex® kit, please search for your analyte of interest.

Livello qualitativo

Reattività contro le specie

rat

Produttore/marchio commerciale

Milliplex®

assay range

accuracy: 103%
(Amylin (Active))

accuracy: 96%
(GLP-1 (Active))

accuracy: 96%
(IL-6)

accuracy: 96%
(MCP-1)

accuracy: 98%
(TNFα)

accuracy: 99%
(C-Peptide 2)

accuracy: 99%
(Insulin)

sensitivity: 1-62 pg/mL
standard curve range: 14-10,000 pg/mL
(Glucagon & TNFα)

standard curve range: 2.7-2,000 pg/mL
(GIP (Total))

standard curve range: 41-30,000 pg/mL
(Amylin (Active) & GLP-1 (Active))

standard curve range: 69-50,000 pg/mL
(C-Peptide 2, IL-6, Insulin, Leptin & MCP-1)

standard curve range: 7-5,000 pg/mL
(Ghrelin (Active), PP & PYY (Total))

tecniche

multiplexing: suitable

Metodo di rivelazione

fluorometric (Luminex xMAP)

Condizioni di spedizione

wet ice

Descrizione generale

Metabolic syndrome is a cluster of conditions, including increased blood pressure, elevated insulin levels, abnormal cholesterol levels and excess body fat around the waist. Key features of metabolic syndrome include insulin resistance, glucose intolerance, hypertension, dyslipidemia, and central obesity.

MILLIPLEX® Rat Metabolic Panel is a 13-plex kit to be used for the simultaneous quantification of any or all of the following analytes in tissue/cell lysate and culture supernatant samples and serum or plasma samples: Amylin (active), C-peptide 2, Active Ghrelin, GIP, GLP-1, Glucagon, IL-6, Insulin, Leptin, MCP-1, PP, PYY, and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

Specificità

Cross Reactivty
No significant cross-reactivity with other hormones tested.

Applicazioni

  • Analytes: Amylin (Active), C-Peptide 2, Ghrelin (Active), GIP (Total), GLP-1 (Active), Glucagon, IL-6, Insulin, Leptin, MCP-1 (CCL2), Pancreatic Polypeptide (PP), PYY (Total), TNFα
  • Recommended Sample Type: Rat serum, plasma, cell/tissue culture supernatants and lysates.
  • NOTE: For specific analytes protease inhibitors should be added upon blood collection: DPPIV for GLP-1 (Active); Protease Inhibitor Cocktail for Amylin (Active); Aprotinin for Glucagon; Serine Protease Inhibitor for Ghrelin (Active)
  • Recommended Sample Dilution: 25 μL per well of neat serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium.
  • Assay Run Time: Overnight (18-20 hours) at 2-8°C
  • Research Category: Metabolism
  • Research Subcategory: Metabolic Disorders, Obesity, Endocrine

Caratteristiche e vantaggi

Design your multiplex kit by choosing available analytes within this panel.

Note legali

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pittogrammi

Skull and crossbonesEnvironment

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1

Rischi supp

Codice della classe di stoccaggio

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


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Maritza J Romero et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(7), 1895-1900 (2016-02-03)
Dyslipidemia associated with triglyceride-rich lipoproteins (TRLs) represents an important residual risk factor for cardiovascular and chronic kidney disease in patients with type 1 diabetes (T1D). Levels of growth hormone (GH) are elevated in T1D, which aggravates both hyperglycemia and dyslipidemia.
Atanu Pal et al.
Diabetes, 64(6), 1941-1950 (2015-01-13)
The antidiabetes effects of Roux-en-Y gastric bypass (RYGB) are well-known, but the underlying mechanisms remain unclear. Isolating the proximal small intestine, and in particular its luminal glucose sensors, from the nutrient stream has been proposed as a critical change, but
Carolina Soares Moura et al.
Food & nutrition research, 61(1), 1290740-1290740 (2017-03-23)
Background: Several physiologically beneficial effects of consuming a whey protein hydrolysate (WPH) have been attributed to the greater availability of bioactive peptides. Aims: The aim was to investigate the effect of four branched-chain amino acid- (BCAA-)containing dipeptides, present in WPH
Sunil Sirohi et al.
Obesity (Silver Spring, Md.), 25(7), 1228-1236 (2017-05-14)
Roux-en-Y gastric bypass (RYGB) surgery reduces appetite and stimulates new onset alcohol misuse; however, the genesis of these behavioral changes is unclear. This study is hypothesized that new onset alcohol intake is a behavioral adaptation that occurs secondary to reduced
Starr Villavasso et al.
Nutrients, 11(11) (2019-11-14)
We have previously shown that 6 weeks of intermittent high-fat diet (Int-HFD) pre-exposure significantly reduced alcohol drinking in rats, providing preliminary evidence of the effectiveness of a dietary intervention in reducing alcohol intake. However, the functional framework and underlying neurobiological

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